Generation of innate immune reporter cells using retroviral transduction

Yamel Cardona Gloria, Eicke Latz, Dominic De Nardo

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

4 Citations (Scopus)

Abstract

Innate immune cells are notoriously difficult to transfect; however, retroviruses can be used to stably integrate genes of interest into the host genome of primary or immortalized immune cells resulting in the generation of reporter cells. Here, we provide a detailed protocol covering the production of retroviruses, retroviral infection of innate immune target cells (including isolation and differentiation of murine bone marrow cells to macrophages), and several methods for enrichment of positively transduced cells.

Original languageEnglish
Title of host publicationInnate Immune Activation
PublisherHumana Press
Pages97-117
Number of pages21
ISBN (Electronic)9781493975198
ISBN (Print)9781493975181
DOIs
Publication statusPublished - 1 Jan 2018
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1714
ISSN (Print)1064-3745

Keywords

  • Bone marrow isolation
  • Bone marrow-derived macrophages
  • Fluorescent protein
  • Infection
  • Innate immune reporter cells
  • Innate immunity
  • Retrovirus
  • Transduction

Cite this

Gloria, Y. C., Latz, E., & De Nardo, D. (2018). Generation of innate immune reporter cells using retroviral transduction. In Innate Immune Activation (pp. 97-117). (Methods in Molecular Biology; Vol. 1714). Humana Press. https://doi.org/10.1007/978-1-4939-7519-8_7