Generation of innate immune reporter cells using retroviral transduction

Yamel Cardona Gloria, Eicke Latz, Dominic De Nardo

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

9 Citations (Scopus)


Innate immune cells are notoriously difficult to transfect; however, retroviruses can be used to stably integrate genes of interest into the host genome of primary or immortalized immune cells resulting in the generation of reporter cells. Here, we provide a detailed protocol covering the production of retroviruses, retroviral infection of innate immune target cells (including isolation and differentiation of murine bone marrow cells to macrophages), and several methods for enrichment of positively transduced cells.

Original languageEnglish
Title of host publicationInnate Immune Activation
PublisherHumana Press
Number of pages21
ISBN (Electronic)9781493975198
ISBN (Print)9781493975181
Publication statusPublished - 1 Jan 2018
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Bone marrow isolation
  • Bone marrow-derived macrophages
  • Fluorescent protein
  • Infection
  • Innate immune reporter cells
  • Innate immunity
  • Retrovirus
  • Transduction

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