High-symmetry lipid nanoparticles with internal bicontinuous cubic phase structure (cubosomes) are prepared from a simple emulsion containing a mixture of a nondigestible lipid (phytantriol) and a digestible short-chained triglyceride using enzymatic lipolysis of the incorporated short-chained triglyceride. The lipolytic products partition away from the nondigestible lipid, resulting in crystallization of the cubic-phase internal structure. Time-resolved small-angle X-ray scattering revealed the kinetics of the disorder-to-order transition, with cryo-transmission electron microscopy showing an absence of liposomes. The new approach offers a new sideways method for the generation of lipid-based nanostructured materials that avoids the problems of top-down and bottom-up approaches.
Fong, W. K., Salentinig, S. J., Prestidge, C. A., Mezzenga, R., Hawley, A., & Boyd, B. J. (2014). Generation of geometrically ordered lipid-based liquid-crystalline nanoparticles using biologically relevant enzymatic processing. Langmuir, 30(19), 5373 - 5377. https://doi.org/10.1021/la5003447