Generation of geometrically ordered lipid-based liquid-crystalline nanoparticles using biologically relevant enzymatic processing

Wye Khay Fong, Stefan Johannes Salentinig, Clive Allan Prestidge, Raffaele Mezzenga, Adrian Hawley, Benjamin James Boyd

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30 Citations (Scopus)


High-symmetry lipid nanoparticles with internal bicontinuous cubic phase structure (cubosomes) are prepared from a simple emulsion containing a mixture of a nondigestible lipid (phytantriol) and a digestible short-chained triglyceride using enzymatic lipolysis of the incorporated short-chained triglyceride. The lipolytic products partition away from the nondigestible lipid, resulting in crystallization of the cubic-phase internal structure. Time-resolved small-angle X-ray scattering revealed the kinetics of the disorder-to-order transition, with cryo-transmission electron microscopy showing an absence of liposomes. The new approach offers a new sideways method for the generation of lipid-based nanostructured materials that avoids the problems of top-down and bottom-up approaches.
Original languageEnglish
Pages (from-to)5373 - 5377
Number of pages5
Issue number19
Publication statusPublished - 2014

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