Generation of four iPSC lines from peripheral blood mononuclear cells (PBMCs) of an attention deficit hyperactivity disorder (ADHD) individual and a healthy sibling in an Australia-Caucasian family

Janette Tong, Kyung Min Lee, Xiaodong Liu, Christian M. Nefzger, Prasidhee Vijayakumar, Ziarih Hawi, Ken C. Pang, Clare L. Parish, Jose M. Polo, Mark A. Bellgrove

Research output: Contribution to journalEditorialOtherpeer-review

2 Citations (Scopus)

Abstract

Peripheral blood mononuclear cells were donated by a male teenager with clinically diagnosed attention deficit hyperactivity disorder (ADHD) under the Diagnostic and Statistical Manual of Mental Disorders IV criteria and his unaffected male sibling. Induced pluripotent stem cells were developed using integration-free Sendai Reprogramming factors containing OCT4, SOX2, KLF4, and c-MYC. All four iPSC lines displayed pluripotent cell morphology, pluripotency-associated factors at the DNA and protein level, alkaline phosphatase enzymatic activity and a male karyotype of 46, XY. All lines had capacity for in vitro differentiation into all the three germ layers. All were negative for Mycoplasma.

Original languageEnglish
Article number101353
Number of pages5
JournalStem Cell Research
Volume34
DOIs
Publication statusPublished - 1 Jan 2019

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