Generation of four iPSC lines from peripheral blood mononuclear cells (PBMCs) of an attention deficit hyperactivity disorder (ADHD) individual and a healthy sibling in an Australia-Caucasian family

Janette Tong, Kyung Min Lee, Xiaodong Liu, Christian M. Nefzger, Prasidhee Vijayakumar, Ziarih Hawi, Ken C. Pang, Clare L. Parish, Jose M. Polo, Mark A. Bellgrove

Research output: Contribution to journalEditorialOtherpeer-review

Abstract

Peripheral blood mononuclear cells were donated by a male teenager with clinically diagnosed attention deficit hyperactivity disorder (ADHD) under the Diagnostic and Statistical Manual of Mental Disorders IV criteria and his unaffected male sibling. Induced pluripotent stem cells were developed using integration-free Sendai Reprogramming factors containing OCT4, SOX2, KLF4, and c-MYC. All four iPSC lines displayed pluripotent cell morphology, pluripotency-associated factors at the DNA and protein level, alkaline phosphatase enzymatic activity and a male karyotype of 46, XY. All lines had capacity for in vitro differentiation into all the three germ layers. All were negative for Mycoplasma.

Original languageEnglish
Article number101353
Number of pages5
JournalStem Cell Research
Volume34
DOIs
Publication statusPublished - 1 Jan 2019

Cite this

@article{69d86123beb2419788621f41face0453,
title = "Generation of four iPSC lines from peripheral blood mononuclear cells (PBMCs) of an attention deficit hyperactivity disorder (ADHD) individual and a healthy sibling in an Australia-Caucasian family",
abstract = "Peripheral blood mononuclear cells were donated by a male teenager with clinically diagnosed attention deficit hyperactivity disorder (ADHD) under the Diagnostic and Statistical Manual of Mental Disorders IV criteria and his unaffected male sibling. Induced pluripotent stem cells were developed using integration-free Sendai Reprogramming factors containing OCT4, SOX2, KLF4, and c-MYC. All four iPSC lines displayed pluripotent cell morphology, pluripotency-associated factors at the DNA and protein level, alkaline phosphatase enzymatic activity and a male karyotype of 46, XY. All lines had capacity for in vitro differentiation into all the three germ layers. All were negative for Mycoplasma.",
author = "Janette Tong and Lee, {Kyung Min} and Xiaodong Liu and Nefzger, {Christian M.} and Prasidhee Vijayakumar and Ziarih Hawi and Pang, {Ken C.} and Parish, {Clare L.} and Polo, {Jose M.} and Bellgrove, {Mark A.}",
year = "2019",
month = "1",
day = "1",
doi = "10.1016/j.scr.2018.11.014",
language = "English",
volume = "34",
journal = "Stem Cell Research",
issn = "1873-5061",
publisher = "Elsevier",

}

Generation of four iPSC lines from peripheral blood mononuclear cells (PBMCs) of an attention deficit hyperactivity disorder (ADHD) individual and a healthy sibling in an Australia-Caucasian family. / Tong, Janette; Lee, Kyung Min; Liu, Xiaodong; Nefzger, Christian M.; Vijayakumar, Prasidhee; Hawi, Ziarih; Pang, Ken C.; Parish, Clare L.; Polo, Jose M.; Bellgrove, Mark A.

In: Stem Cell Research, Vol. 34, 101353, 01.01.2019.

Research output: Contribution to journalEditorialOtherpeer-review

TY - JOUR

T1 - Generation of four iPSC lines from peripheral blood mononuclear cells (PBMCs) of an attention deficit hyperactivity disorder (ADHD) individual and a healthy sibling in an Australia-Caucasian family

AU - Tong, Janette

AU - Lee, Kyung Min

AU - Liu, Xiaodong

AU - Nefzger, Christian M.

AU - Vijayakumar, Prasidhee

AU - Hawi, Ziarih

AU - Pang, Ken C.

AU - Parish, Clare L.

AU - Polo, Jose M.

AU - Bellgrove, Mark A.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Peripheral blood mononuclear cells were donated by a male teenager with clinically diagnosed attention deficit hyperactivity disorder (ADHD) under the Diagnostic and Statistical Manual of Mental Disorders IV criteria and his unaffected male sibling. Induced pluripotent stem cells were developed using integration-free Sendai Reprogramming factors containing OCT4, SOX2, KLF4, and c-MYC. All four iPSC lines displayed pluripotent cell morphology, pluripotency-associated factors at the DNA and protein level, alkaline phosphatase enzymatic activity and a male karyotype of 46, XY. All lines had capacity for in vitro differentiation into all the three germ layers. All were negative for Mycoplasma.

AB - Peripheral blood mononuclear cells were donated by a male teenager with clinically diagnosed attention deficit hyperactivity disorder (ADHD) under the Diagnostic and Statistical Manual of Mental Disorders IV criteria and his unaffected male sibling. Induced pluripotent stem cells were developed using integration-free Sendai Reprogramming factors containing OCT4, SOX2, KLF4, and c-MYC. All four iPSC lines displayed pluripotent cell morphology, pluripotency-associated factors at the DNA and protein level, alkaline phosphatase enzymatic activity and a male karyotype of 46, XY. All lines had capacity for in vitro differentiation into all the three germ layers. All were negative for Mycoplasma.

UR - http://www.scopus.com/inward/record.url?scp=85059530266&partnerID=8YFLogxK

U2 - 10.1016/j.scr.2018.11.014

DO - 10.1016/j.scr.2018.11.014

M3 - Editorial

VL - 34

JO - Stem Cell Research

JF - Stem Cell Research

SN - 1873-5061

M1 - 101353

ER -