Abstract
Peripheral blood mononuclear cells were donated by a male teenager with clinically diagnosed attention deficit hyperactivity disorder (ADHD) under the Diagnostic and Statistical Manual of Mental Disorders IV criteria and his unaffected male sibling. Induced pluripotent stem cells were developed using integration-free Sendai Reprogramming factors containing OCT4, SOX2, KLF4, and c-MYC. All four iPSC lines displayed pluripotent cell morphology, pluripotency-associated factors at the DNA and protein level, alkaline phosphatase enzymatic activity and a male karyotype of 46, XY. All lines had capacity for in vitro differentiation into all the three germ layers. All were negative for Mycoplasma.
| Original language | English |
|---|---|
| Article number | 101353 |
| Number of pages | 5 |
| Journal | Stem Cell Research |
| Volume | 34 |
| DOIs | |
| Publication status | Published - 1 Jan 2019 |
Cite this
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Generation of four iPSC lines from peripheral blood mononuclear cells (PBMCs) of an attention deficit hyperactivity disorder (ADHD) individual and a healthy sibling in an Australia-Caucasian family. / Tong, Janette; Lee, Kyung Min; Liu, Xiaodong; Nefzger, Christian M.; Vijayakumar, Prasidhee; Hawi, Ziarih; Pang, Ken C.; Parish, Clare L.; Polo, Jose M.; Bellgrove, Mark A.
In: Stem Cell Research, Vol. 34, 101353, 01.01.2019.Research output: Contribution to journal › Editorial › Other › peer-review
TY - JOUR
T1 - Generation of four iPSC lines from peripheral blood mononuclear cells (PBMCs) of an attention deficit hyperactivity disorder (ADHD) individual and a healthy sibling in an Australia-Caucasian family
AU - Tong, Janette
AU - Lee, Kyung Min
AU - Liu, Xiaodong
AU - Nefzger, Christian M.
AU - Vijayakumar, Prasidhee
AU - Hawi, Ziarih
AU - Pang, Ken C.
AU - Parish, Clare L.
AU - Polo, Jose M.
AU - Bellgrove, Mark A.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Peripheral blood mononuclear cells were donated by a male teenager with clinically diagnosed attention deficit hyperactivity disorder (ADHD) under the Diagnostic and Statistical Manual of Mental Disorders IV criteria and his unaffected male sibling. Induced pluripotent stem cells were developed using integration-free Sendai Reprogramming factors containing OCT4, SOX2, KLF4, and c-MYC. All four iPSC lines displayed pluripotent cell morphology, pluripotency-associated factors at the DNA and protein level, alkaline phosphatase enzymatic activity and a male karyotype of 46, XY. All lines had capacity for in vitro differentiation into all the three germ layers. All were negative for Mycoplasma.
AB - Peripheral blood mononuclear cells were donated by a male teenager with clinically diagnosed attention deficit hyperactivity disorder (ADHD) under the Diagnostic and Statistical Manual of Mental Disorders IV criteria and his unaffected male sibling. Induced pluripotent stem cells were developed using integration-free Sendai Reprogramming factors containing OCT4, SOX2, KLF4, and c-MYC. All four iPSC lines displayed pluripotent cell morphology, pluripotency-associated factors at the DNA and protein level, alkaline phosphatase enzymatic activity and a male karyotype of 46, XY. All lines had capacity for in vitro differentiation into all the three germ layers. All were negative for Mycoplasma.
UR - http://www.scopus.com/inward/record.url?scp=85059530266&partnerID=8YFLogxK
U2 - 10.1016/j.scr.2018.11.014
DO - 10.1016/j.scr.2018.11.014
M3 - Editorial
VL - 34
JO - Stem Cell Research
JF - Stem Cell Research
SN - 1873-5061
M1 - 101353
ER -