Generation of a non-leaky heat shock-inducible Cre line for conditional Cre/lox strategies in zebrafish

Stefan Hans, Dorian Freudenreich, Michaela Geffarth, Jan V Kaslin, Anja Machate, Michael Brand

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Cre-mediated site-specific recombination has emerged as an indispensable tool for the precise manipulation of the mammalian genome. Recently, we showed that Cre is also highly efficient in zebrafish and temporal control of recombination can be achieved by using the ligand-inducible CreER(T2). Previous attempts have been made to control recombination by using the temperature inducible hsp70l promoter to conditionally drive the expression of Cre or EGFP-Cre, respectively. However, in this study we demonstrate that the hsp70l promoter possesses a basal leakiness resulting in Cre-mediated recombination even at permissive temperatures. In order to prevent non-conditional recombination, we combined the hsp70l promoter with a mCherry-tagged ligand-inducible CreER(T2). At permissive temperatures and in the absence of the ligand tamoxifen (TAM), no non-conditional recombination is observed indicating tight regulation of CreER(T2). Instead, comprehensive site-specific recombination is mediated following heat induction and administration of TAM.
Original languageEnglish
Pages (from-to)108 - 115
Number of pages8
JournalDevelopmental Dynamics
Issue number1
Publication statusPublished - 2011
Externally publishedYes

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