Generation and functional characterization of murine mammary organoids

Hon Yan Kelvin Yip, Antonella Papa

Research output: Contribution to journalArticleOtherpeer-review

3 Citations (Scopus)


3D cultures of mammary epithelial cells purified from murine models provide a unique resource to study genetically defined breast cancer and response to targeted therapies. Here, we describe step-by-step experimental procedures for the successful establishment of murine mammary organoid lines isolated from mammary glands or mammary tumors driven by mutations in components of the PI3K pathway. These detailed protocols also include procedures to perform assays that can be adopted to screen response to drug treatments and to inform better therapies. For details on potential applications and use of this protocol, please refer to Yip et al. (2020).

Original languageEnglish
Article number100765
Number of pages24
JournalSTAR Protocols
Issue number3
Publication statusPublished - 17 Sept 2021


  • Cancer
  • Cell culture
  • Cell isolation
  • Cell-based Assays
  • Organoids

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