Generation and functional characterization of murine mammary organoids

Hon Yan Kelvin Yip; Antonella Papa

doi:10.1016/j.xpro.2021.100765

Generation and functional characterization of murine mammary organoids

Hon Yan Kelvin Yip, Antonella Papa

Research output: Contribution to journal › Article › Other › peer-review

6 Citations (Scopus)

Abstract

3D cultures of mammary epithelial cells purified from murine models provide a unique resource to study genetically defined breast cancer and response to targeted therapies. Here, we describe step-by-step experimental procedures for the successful establishment of murine mammary organoid lines isolated from mammary glands or mammary tumors driven by mutations in components of the PI3K pathway. These detailed protocols also include procedures to perform assays that can be adopted to screen response to drug treatments and to inform better therapies. For details on potential applications and use of this protocol, please refer to Yip et al. (2020).

Original language	English
Article number	100765
Number of pages	24
Journal	STAR Protocols
Volume	2
Issue number	3
DOIs	https://doi.org/10.1016/j.xpro.2021.100765
Publication status	Published - 17 Sept 2021

Keywords

Cancer
Cell culture
Cell isolation
Cell-based Assays
Organoids

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.xpro.2021.100765Licence: CC BY-NC-ND

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title = "Generation and functional characterization of murine mammary organoids",

abstract = "3D cultures of mammary epithelial cells purified from murine models provide a unique resource to study genetically defined breast cancer and response to targeted therapies. Here, we describe step-by-step experimental procedures for the successful establishment of murine mammary organoid lines isolated from mammary glands or mammary tumors driven by mutations in components of the PI3K pathway. These detailed protocols also include procedures to perform assays that can be adopted to screen response to drug treatments and to inform better therapies. For details on potential applications and use of this protocol, please refer to Yip et al. (2020).",

keywords = "Cancer, Cell culture, Cell isolation, Cell-based Assays, Organoids",

author = "Yip, {Hon Yan Kelvin} and Antonella Papa",

note = "Funding Information: The authors would like to thank the Monash Animal Research Platform for animal care and the Monash Organoid Program, the Monash Micro Imaging Facility, Biochemistry Imaging Facility, and the Monash FlowCore for technical assistance and reagents. Graphical abstract and Figure 6 were created with BioRender.com. A.P. is supported by a Victorian Cancer Agency (VCA) Mid-Career Research Fellowship (MCRF20027). Conceptualization, H.Y.K.Y. and A.P.; methodology, H.Y.K.Y. and A.P.; formal analysis, H.Y.K.Y.; investigation, H.Y.K.Y. and A.P.; resources, A.P.; data curation, H.Y.K.Y.; writing, H.Y.K.Y. and A.P.; funding acquisition, A.P. The authors declare no competing interests. Funding Information: The authors would like to thank the Monash Animal Research Platform for animal care and the Monash Organoid Program, the Monash Micro Imaging Facility, Biochemistry Imaging Facility, and the Monash FlowCore for technical assistance and reagents. Graphical abstract and Figure 6 were created with BioRender.com . A.P. is supported by a Victorian Cancer Agency (VCA) Mid-Career Research Fellowship ( MCRF20027 ). Publisher Copyright: {\textcopyright} 2021 The Author(s)",

year = "2021",

month = sep,

day = "17",

doi = "10.1016/j.xpro.2021.100765",

language = "English",

volume = "2",

journal = "STAR Protocols",

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T1 - Generation and functional characterization of murine mammary organoids

AU - Yip, Hon Yan Kelvin

AU - Papa, Antonella

N1 - Funding Information: The authors would like to thank the Monash Animal Research Platform for animal care and the Monash Organoid Program, the Monash Micro Imaging Facility, Biochemistry Imaging Facility, and the Monash FlowCore for technical assistance and reagents. Graphical abstract and Figure 6 were created with BioRender.com. A.P. is supported by a Victorian Cancer Agency (VCA) Mid-Career Research Fellowship (MCRF20027). Conceptualization, H.Y.K.Y. and A.P.; methodology, H.Y.K.Y. and A.P.; formal analysis, H.Y.K.Y.; investigation, H.Y.K.Y. and A.P.; resources, A.P.; data curation, H.Y.K.Y.; writing, H.Y.K.Y. and A.P.; funding acquisition, A.P. The authors declare no competing interests. Funding Information: The authors would like to thank the Monash Animal Research Platform for animal care and the Monash Organoid Program, the Monash Micro Imaging Facility, Biochemistry Imaging Facility, and the Monash FlowCore for technical assistance and reagents. Graphical abstract and Figure 6 were created with BioRender.com . A.P. is supported by a Victorian Cancer Agency (VCA) Mid-Career Research Fellowship ( MCRF20027 ). Publisher Copyright: © 2021 The Author(s)

PY - 2021/9/17

Y1 - 2021/9/17

N2 - 3D cultures of mammary epithelial cells purified from murine models provide a unique resource to study genetically defined breast cancer and response to targeted therapies. Here, we describe step-by-step experimental procedures for the successful establishment of murine mammary organoid lines isolated from mammary glands or mammary tumors driven by mutations in components of the PI3K pathway. These detailed protocols also include procedures to perform assays that can be adopted to screen response to drug treatments and to inform better therapies. For details on potential applications and use of this protocol, please refer to Yip et al. (2020).

AB - 3D cultures of mammary epithelial cells purified from murine models provide a unique resource to study genetically defined breast cancer and response to targeted therapies. Here, we describe step-by-step experimental procedures for the successful establishment of murine mammary organoid lines isolated from mammary glands or mammary tumors driven by mutations in components of the PI3K pathway. These detailed protocols also include procedures to perform assays that can be adopted to screen response to drug treatments and to inform better therapies. For details on potential applications and use of this protocol, please refer to Yip et al. (2020).

KW - Cancer

KW - Cell culture

KW - Cell isolation

KW - Cell-based Assays

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ER -

Generation and functional characterization of murine mammary organoids

Abstract

Keywords

UN SDGs

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Optimising combination therapies for breast cancer

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Generation and functional characterization of murine mammary organoids

Abstract

Keywords

UN SDGs

Access to Document

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Projects

Optimising combination therapies for breast cancer

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