Generation and characterization of mice with null mutation of the chloride intracellular channel 1 gene

Min Qiu; Lele Jiang; Klaus I Matthaei; Simone Marianne Schoenwaelder; Tamara Kuffner; Pierre Mangin; Joanne E Joseph; Joyce Low; David Connor; Stella M Valenzuela; Paul MG Curmi; Louise J Brown; Martyn Mahaut-Smith; Shaun Jackson; Samuel N Breit

doi:10.1002/dvg.20590

Generation and characterization of mice with null mutation of the chloride intracellular channel 1 gene

Min Qiu, Lele Jiang, Klaus I Matthaei, Simone Marianne Schoenwaelder, Tamara Kuffner, Pierre Mangin, Joanne E Joseph, Joyce Low, David Connor, Stella M Valenzuela, Paul MG Curmi, Louise J Brown, Martyn Mahaut-Smith, Shaun Jackson, Samuel N Breit

Australian Centre for Blood Diseases

Research output: Contribution to journal › Article › Research › peer-review

34 Citations (Scopus)

Abstract

To study the physiological and biological role of CLIC1 in vivo, we undertook conditional gene targeting to engineer Clic1 gene knock-out mice. This represents creation of the first gene knock-out of a vertebrate CLIC protein family member. We first generated a Clic1 Knock-in (Clic1FN) allele, followed by Clic1 knock-out (Clic1a??/a??) mice by crossing Clic1FN allele with TNAP-cre mice, resulting in germline gene deletion through Cre-mediated recombination. Mice heterozygous or homozygous for these alleles are viable and fertile and appear normal.

Original language	English
Pages (from-to)	127 - 136
Number of pages	10
Journal	genesis: The Journal of Genetics and Development
Volume	48
Issue number	2
DOIs	https://doi.org/10.1002/dvg.20590
Publication status	Published - 2010

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Qiu, M., Jiang, L., Matthaei, K. I., Schoenwaelder, S. M., Kuffner, T., Mangin, P., Joseph, J. E., Low, J., Connor, D., Valenzuela, S. M., Curmi, P. MG., Brown, L. J., Mahaut-Smith, M., Jackson, S., & Breit, S. N. (2010). Generation and characterization of mice with null mutation of the chloride intracellular channel 1 gene. genesis: The Journal of Genetics and Development, 48(2), 127 - 136. https://doi.org/10.1002/dvg.20590

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title = "Generation and characterization of mice with null mutation of the chloride intracellular channel 1 gene",

abstract = "To study the physiological and biological role of CLIC1 in vivo, we undertook conditional gene targeting to engineer Clic1 gene knock-out mice. This represents creation of the first gene knock-out of a vertebrate CLIC protein family member. We first generated a Clic1 Knock-in (Clic1FN) allele, followed by Clic1 knock-out (Clic1a??/a??) mice by crossing Clic1FN allele with TNAP-cre mice, resulting in germline gene deletion through Cre-mediated recombination. Mice heterozygous or homozygous for these alleles are viable and fertile and appear normal.",

author = "Min Qiu and Lele Jiang and Matthaei, {Klaus I} and Schoenwaelder, {Simone Marianne} and Tamara Kuffner and Pierre Mangin and Joseph, {Joanne E} and Joyce Low and David Connor and Valenzuela, {Stella M} and Curmi, {Paul MG} and Brown, {Louise J} and Martyn Mahaut-Smith and Shaun Jackson and Breit, {Samuel N}",

year = "2010",

doi = "10.1002/dvg.20590",

language = "English",

volume = "48",

pages = "127 -- 136",

journal = "genesis: The Journal of Genetics and Development",

issn = "1526-954X",

publisher = "John Wiley & Sons",

number = "2",

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Qiu, M, Jiang, L, Matthaei, KI, Schoenwaelder, SM, Kuffner, T, Mangin, P, Joseph, JE, Low, J, Connor, D, Valenzuela, SM, Curmi, PMG, Brown, LJ, Mahaut-Smith, M, Jackson, S & Breit, SN 2010, 'Generation and characterization of mice with null mutation of the chloride intracellular channel 1 gene', genesis: The Journal of Genetics and Development, vol. 48, no. 2, pp. 127 - 136. https://doi.org/10.1002/dvg.20590

TY - JOUR

T1 - Generation and characterization of mice with null mutation of the chloride intracellular channel 1 gene

AU - Qiu, Min

AU - Jiang, Lele

AU - Matthaei, Klaus I

AU - Schoenwaelder, Simone Marianne

AU - Kuffner, Tamara

AU - Mangin, Pierre

AU - Joseph, Joanne E

AU - Low, Joyce

AU - Connor, David

AU - Valenzuela, Stella M

AU - Curmi, Paul MG

AU - Brown, Louise J

AU - Mahaut-Smith, Martyn

AU - Jackson, Shaun

AU - Breit, Samuel N

PY - 2010

Y1 - 2010

N2 - To study the physiological and biological role of CLIC1 in vivo, we undertook conditional gene targeting to engineer Clic1 gene knock-out mice. This represents creation of the first gene knock-out of a vertebrate CLIC protein family member. We first generated a Clic1 Knock-in (Clic1FN) allele, followed by Clic1 knock-out (Clic1a??/a??) mice by crossing Clic1FN allele with TNAP-cre mice, resulting in germline gene deletion through Cre-mediated recombination. Mice heterozygous or homozygous for these alleles are viable and fertile and appear normal.

AB - To study the physiological and biological role of CLIC1 in vivo, we undertook conditional gene targeting to engineer Clic1 gene knock-out mice. This represents creation of the first gene knock-out of a vertebrate CLIC protein family member. We first generated a Clic1 Knock-in (Clic1FN) allele, followed by Clic1 knock-out (Clic1a??/a??) mice by crossing Clic1FN allele with TNAP-cre mice, resulting in germline gene deletion through Cre-mediated recombination. Mice heterozygous or homozygous for these alleles are viable and fertile and appear normal.

UR - http://onlinelibrary.wiley.com/doi/10.1002/dvg.20590/pdf

U2 - 10.1002/dvg.20590

DO - 10.1002/dvg.20590

M3 - Article

SN - 1526-954X

VL - 48

SP - 127

EP - 136

JO - genesis: The Journal of Genetics and Development

JF - genesis: The Journal of Genetics and Development

IS - 2

ER -

Generation and characterization of mice with null mutation of the chloride intracellular channel 1 gene

Abstract

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