Generation and characterization of mice with null mutation of the chloride intracellular channel 1 gene

Min Qiu, Lele Jiang, Klaus I Matthaei, Simone Marianne Schoenwaelder, Tamara Kuffner, Pierre Mangin, Joanne E Joseph, Joyce Low, David Connor, Stella M Valenzuela, Paul MG Curmi, Louise J Brown, Martyn Mahaut-Smith, Shaun Jackson, Samuel N Breit

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28 Citations (Scopus)

Abstract

To study the physiological and biological role of CLIC1 in vivo, we undertook conditional gene targeting to engineer Clic1 gene knock-out mice. This represents creation of the first gene knock-out of a vertebrate CLIC protein family member. We first generated a Clic1 Knock-in (Clic1FN) allele, followed by Clic1 knock-out (Clic1a??/a??) mice by crossing Clic1FN allele with TNAP-cre mice, resulting in germline gene deletion through Cre-mediated recombination. Mice heterozygous or homozygous for these alleles are viable and fertile and appear normal.
Original languageEnglish
Pages (from-to)127 - 136
Number of pages10
Journalgenesis: The Journal of Genetics and Development
Volume48
Issue number2
DOIs
Publication statusPublished - 2010

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