Abstract
To study the physiological and biological role of CLIC1 in vivo, we undertook conditional gene targeting to engineer Clic1 gene knock-out mice. This represents creation of the first gene knock-out of a vertebrate CLIC protein family member. We first generated a Clic1 Knock-in (Clic1FN) allele, followed by Clic1 knock-out (Clic1a??/a??) mice by crossing Clic1FN allele with TNAP-cre mice, resulting in germline gene deletion through Cre-mediated recombination. Mice heterozygous or homozygous for these alleles are viable and fertile and appear normal.
Original language | English |
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Pages (from-to) | 127 - 136 |
Number of pages | 10 |
Journal | genesis: The Journal of Genetics and Development |
Volume | 48 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2010 |