Generation 3 PAMAM dendrimer TAMRA conjugates containing precise dye/dendrimer ratios

Janet Manono, Casey A. Dougherty, Kirsten Jones, Joshua DeMuth, Mark M. Banaszak Holl, Stassi DiMaggio

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6 Citations (Scopus)

Abstract

The synthesis, isolation, and characterization of generation 3 poly(amidoamine) (G3 PAMAM) dendrimer containing precise ratios of 5-carboxytetramethylrhodamine succinimidyl ester (TAMRA) dye (n=1-3) per polymer particle are reported. Stochastic conjugation of TAMRA dye to the dendrimer was followed by separation into precise dye-polymer ratios using rp-HPLC. The isolated materials were characterized by rp-UPLC, MALDI-TOF-MS, and 1H NMR spectroscopy, UV-vis, and fluorescence spectroscopies.

Original languageEnglish
Pages (from-to)86-92
Number of pages7
JournalMaterials Today Communications
Volume4
DOIs
Publication statusPublished - 1 Sep 2015
Externally publishedYes

Keywords

  • Fluorescent dye
  • PAMAM
  • Poly(amidoamine) dendrimer
  • Rp-HPLC

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