TY - JOUR
T1 - Generation 3 PAMAM dendrimer TAMRA conjugates containing precise dye/dendrimer ratios
AU - Manono, Janet
AU - Dougherty, Casey A.
AU - Jones, Kirsten
AU - DeMuth, Joshua
AU - Holl, Mark M. Banaszak
AU - DiMaggio, Stassi
PY - 2015/9/1
Y1 - 2015/9/1
N2 - The synthesis, isolation, and characterization of generation 3 poly(amidoamine) (G3 PAMAM) dendrimer containing precise ratios of 5-carboxytetramethylrhodamine succinimidyl ester (TAMRA) dye (n=1-3) per polymer particle are reported. Stochastic conjugation of TAMRA dye to the dendrimer was followed by separation into precise dye-polymer ratios using rp-HPLC. The isolated materials were characterized by rp-UPLC, MALDI-TOF-MS, and 1H NMR spectroscopy, UV-vis, and fluorescence spectroscopies.
AB - The synthesis, isolation, and characterization of generation 3 poly(amidoamine) (G3 PAMAM) dendrimer containing precise ratios of 5-carboxytetramethylrhodamine succinimidyl ester (TAMRA) dye (n=1-3) per polymer particle are reported. Stochastic conjugation of TAMRA dye to the dendrimer was followed by separation into precise dye-polymer ratios using rp-HPLC. The isolated materials were characterized by rp-UPLC, MALDI-TOF-MS, and 1H NMR spectroscopy, UV-vis, and fluorescence spectroscopies.
KW - Fluorescent dye
KW - PAMAM
KW - Poly(amidoamine) dendrimer
KW - Rp-HPLC
UR - http://www.scopus.com/inward/record.url?scp=84933544928&partnerID=8YFLogxK
U2 - 10.1016/j.mtcomm.2015.06.006
DO - 10.1016/j.mtcomm.2015.06.006
M3 - Article
C2 - 26549978
AN - SCOPUS:84933544928
VL - 4
SP - 86
EP - 92
JO - Materials Today Communications
JF - Materials Today Communications
SN - 2352-4928
ER -