Abstract
The synthesis, isolation, and characterization of generation 3 poly(amidoamine) (G3 PAMAM) dendrimer containing precise ratios of 5-carboxytetramethylrhodamine succinimidyl ester (TAMRA) dye (n=1-3) per polymer particle are reported. Stochastic conjugation of TAMRA dye to the dendrimer was followed by separation into precise dye-polymer ratios using rp-HPLC. The isolated materials were characterized by rp-UPLC, MALDI-TOF-MS, and 1H NMR spectroscopy, UV-vis, and fluorescence spectroscopies.
| Original language | English |
|---|---|
| Pages (from-to) | 86-92 |
| Number of pages | 7 |
| Journal | Materials Today Communications |
| Volume | 4 |
| DOIs | |
| Publication status | Published - 1 Sep 2015 |
| Externally published | Yes |
Keywords
- Fluorescent dye
- PAMAM
- Poly(amidoamine) dendrimer
- Rp-HPLC
Cite this
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Generation 3 PAMAM dendrimer TAMRA conjugates containing precise dye/dendrimer ratios. / Manono, Janet; Dougherty, Casey A.; Jones, Kirsten; DeMuth, Joshua; Holl, Mark M. Banaszak; DiMaggio, Stassi.
In: Materials Today Communications, Vol. 4, 01.09.2015, p. 86-92.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Generation 3 PAMAM dendrimer TAMRA conjugates containing precise dye/dendrimer ratios
AU - Manono, Janet
AU - Dougherty, Casey A.
AU - Jones, Kirsten
AU - DeMuth, Joshua
AU - Holl, Mark M. Banaszak
AU - DiMaggio, Stassi
PY - 2015/9/1
Y1 - 2015/9/1
N2 - The synthesis, isolation, and characterization of generation 3 poly(amidoamine) (G3 PAMAM) dendrimer containing precise ratios of 5-carboxytetramethylrhodamine succinimidyl ester (TAMRA) dye (n=1-3) per polymer particle are reported. Stochastic conjugation of TAMRA dye to the dendrimer was followed by separation into precise dye-polymer ratios using rp-HPLC. The isolated materials were characterized by rp-UPLC, MALDI-TOF-MS, and 1H NMR spectroscopy, UV-vis, and fluorescence spectroscopies.
AB - The synthesis, isolation, and characterization of generation 3 poly(amidoamine) (G3 PAMAM) dendrimer containing precise ratios of 5-carboxytetramethylrhodamine succinimidyl ester (TAMRA) dye (n=1-3) per polymer particle are reported. Stochastic conjugation of TAMRA dye to the dendrimer was followed by separation into precise dye-polymer ratios using rp-HPLC. The isolated materials were characterized by rp-UPLC, MALDI-TOF-MS, and 1H NMR spectroscopy, UV-vis, and fluorescence spectroscopies.
KW - Fluorescent dye
KW - PAMAM
KW - Poly(amidoamine) dendrimer
KW - Rp-HPLC
UR - http://www.scopus.com/inward/record.url?scp=84933544928&partnerID=8YFLogxK
U2 - 10.1016/j.mtcomm.2015.06.006
DO - 10.1016/j.mtcomm.2015.06.006
M3 - Article
VL - 4
SP - 86
EP - 92
JO - Materials Today Communications
JF - Materials Today Communications
SN - 2352-4928
ER -