Gene-trap-based target site for Cre-mediated transgenic insertion

Nathalie Hardouin, Andras Nagy

Research output: Contribution to journalArticleResearchpeer-review

30 Citations (Scopus)

Abstract

There is an increasing need for tissue-specific gene expression regulatory elements to study normal and disease development in the mouse. However, the cloning and characterization of these elements are time- consuming and costly. Thus, there is a particular need to be able to identify gene expression patterns without having to clone the promoter elements. Gene- trap strategies identify expression patterns assigned for endogenous genes using reporters, such as LacZ (Gossler et al., 1989; Skarnes, 1990) or green fluorescent protein (GFP) (Ishida and Leder, 1999; Zheng and Hughes, 1999). The gene-trap vector randomly inserts into the genome and 'steals' regulatory elements for the reporter. Here we describe an improved gene-trap strategy, which allows an efficient Cre recombinase-mediated insertion of any transgene into the trapped loci as a post-integrational modification and links the expression of the transgene to that of the reporter. (C) 2000 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)245-252
Number of pages8
Journalgenesis: The Journal of Genetics and Development
Volume26
Issue number4
DOIs
Publication statusPublished - Apr 2000
Externally publishedYes

Keywords

  • Cre recombinase
  • Define site transgene insertion
  • Gene trap
  • Transgene replacement

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