Gene microarrays reveal extensive differential gene expression in both CD4+ and CD8+ type 1 and type 2 T cells

T. Chtanova, R. A. Kemp, A. P.R. Sutherland, F. Ronchese, C. R. Mackay

Research output: Contribution to journalArticleResearchpeer-review

116 Citations (Scopus)

Abstract

An important subdivision of effector T cells can be made based on patterns of cytokine production and functional programs. Type 1 T cells produce IFN-γ and protect against viral pathogens, whereas type 2 cells produce cytokines such as IL-4 and IL-5 and protect against large extracellular parasites. Both CD4+ and CD8+ T cells can be polarized into type 1 or type 2 cytokine-secreting cells, suggesting that both populations play a regulatory role in immune responses. In this study, we used high-density oligonucleotide arrays to produce a comprehensive picture of gene expression in murine CD4+ Th1 and Th2 cells, as well as CD8+ type 1 and type 2 T cells. Polarized type 1 and 2 cells transcribed mRNA for an unexpectedly large number of genes, most of which were expressed in a similar fashion between type 1 and type 2 cells. However, > 100 differentially expressed genes were identified for both the CD4+ and CD8+ type 1 and 2 subsets, many of which have not been associated with T cell polarization. These genes included cytokines, transcription factors, molecules involved in cell migration, as well as genes with unknown function. The program for type 1 or type 2 polarization was similar for CD4+ and CD8+ cells, since gene expression patterns were roughly the same. The expression of select genes was confirmed using real-time PCR. The identification of genes associated with T cell polarization may give important insights into functional and phenotypic differences between effector T cell subsets and their role in normal responses and inflammatory disease.

Original languageEnglish
Pages (from-to)3057-3063
Number of pages7
JournalJournal of Immunology
Volume167
Issue number6
DOIs
Publication statusPublished - 15 Sep 2001
Externally publishedYes

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