TY - JOUR
T1 - Gene microarrays reveal extensive differential gene expression in both CD4+ and CD8+ type 1 and type 2 T cells
AU - Chtanova, T.
AU - Kemp, R. A.
AU - Sutherland, A. P.R.
AU - Ronchese, F.
AU - Mackay, C. R.
PY - 2001/9/15
Y1 - 2001/9/15
N2 - An important subdivision of effector T cells can be made based on patterns of cytokine production and functional programs. Type 1 T cells produce IFN-γ and protect against viral pathogens, whereas type 2 cells produce cytokines such as IL-4 and IL-5 and protect against large extracellular parasites. Both CD4+ and CD8+ T cells can be polarized into type 1 or type 2 cytokine-secreting cells, suggesting that both populations play a regulatory role in immune responses. In this study, we used high-density oligonucleotide arrays to produce a comprehensive picture of gene expression in murine CD4+ Th1 and Th2 cells, as well as CD8+ type 1 and type 2 T cells. Polarized type 1 and 2 cells transcribed mRNA for an unexpectedly large number of genes, most of which were expressed in a similar fashion between type 1 and type 2 cells. However, > 100 differentially expressed genes were identified for both the CD4+ and CD8+ type 1 and 2 subsets, many of which have not been associated with T cell polarization. These genes included cytokines, transcription factors, molecules involved in cell migration, as well as genes with unknown function. The program for type 1 or type 2 polarization was similar for CD4+ and CD8+ cells, since gene expression patterns were roughly the same. The expression of select genes was confirmed using real-time PCR. The identification of genes associated with T cell polarization may give important insights into functional and phenotypic differences between effector T cell subsets and their role in normal responses and inflammatory disease.
AB - An important subdivision of effector T cells can be made based on patterns of cytokine production and functional programs. Type 1 T cells produce IFN-γ and protect against viral pathogens, whereas type 2 cells produce cytokines such as IL-4 and IL-5 and protect against large extracellular parasites. Both CD4+ and CD8+ T cells can be polarized into type 1 or type 2 cytokine-secreting cells, suggesting that both populations play a regulatory role in immune responses. In this study, we used high-density oligonucleotide arrays to produce a comprehensive picture of gene expression in murine CD4+ Th1 and Th2 cells, as well as CD8+ type 1 and type 2 T cells. Polarized type 1 and 2 cells transcribed mRNA for an unexpectedly large number of genes, most of which were expressed in a similar fashion between type 1 and type 2 cells. However, > 100 differentially expressed genes were identified for both the CD4+ and CD8+ type 1 and 2 subsets, many of which have not been associated with T cell polarization. These genes included cytokines, transcription factors, molecules involved in cell migration, as well as genes with unknown function. The program for type 1 or type 2 polarization was similar for CD4+ and CD8+ cells, since gene expression patterns were roughly the same. The expression of select genes was confirmed using real-time PCR. The identification of genes associated with T cell polarization may give important insights into functional and phenotypic differences between effector T cell subsets and their role in normal responses and inflammatory disease.
UR - http://www.scopus.com/inward/record.url?scp=0035884779&partnerID=8YFLogxK
U2 - 10.4049/jimmunol.167.6.3057
DO - 10.4049/jimmunol.167.6.3057
M3 - Article
C2 - 11544289
AN - SCOPUS:0035884779
VL - 167
SP - 3057
EP - 3063
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 6
ER -