Gas chromatography mass spectrometry analysis of volatile compounds from lignosus rhinocerus (Tiger milk mushroom)

M. Johnathan, A. A. Nurul, M. F. Ezumi, S. H. Gan

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4 Citations (Scopus)


Lignosus rhinocerus "Tiger milk mushroom" is used by the indigenous communities in Malaysia as an antipruritic agent and general tonic for treating fever, cough and other diseases. Currently, no studies have reported the constituents of L. rhinocerus, including the volatile constituents. The analysis of the composition of L. rhinocerus would provide a basis for further research on the potential of this mushroom, not only for its nutritional value but also for its medicinal properties. In this study, we aim to identify the volatile components present in L. rhinocerus using gas chromatography mass spectrometry (GCMS). Cultivated dried mushroom powder (1 g) of L. rhinocerus was continuously extracted using five types of solvents with different polarities, such as non-polar solvents petroleum ether and hexane and the polar solvents diethyl ether, ethyl acetate and methanol. Each extraction was individually injected into the GCMS system in duplicate. Overall, forty-four constituents were identified from various groups. Compounds from the fatty acid group were the most predominant (68.58%) followed by fatty acid esters (10.18%), sterols (6.26%), amides (5.76%), carboxylic acids (3.01%), alcohols (1.64%), alkanes (1.26%) and ketones (1.3%). The most common constituent was linoleic acid (49.39%), followed by palmitic acid (11.29%) and linolelaidic acid methyl ester (7.4%) indicating that the eight-carbon atoms components typical in many mushroom species seem to play a minor role in the volatiles of L. rhinocerus. Methanol extracted the greatest number of volatile compounds from L. rhinocerus. Our study is the first to report on the volatile constituents of L. rhinocerus, which is found to be rich in linoleic acid (octadecadienoic acid) and palmitic acid (hexadecanoic acid).

Original languageEnglish
Pages (from-to)5-16
Number of pages12
JournalResearch Journal of Pharmaceutical, Biological and Chemical Sciences
Issue number4
Publication statusPublished - Jul 2016
Externally publishedYes


  • GCMS
  • L. rhinocerus
  • Volatile composition

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