Ganglion cell assessment in rodents with retinal degeneration

Erica L. Fletcher, Ursula Greferath, Susmita Saha, Emily E. Anderson, Kirstan A. Vessey

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

2 Citations (Scopus)

Abstract

Analysis of how retinal ganglion cells change in retinal degeneration is critical for evaluating the potential of photoreceptor restorative therapies. Immunocytochemistry in combination with image analysis provides a way for quantifying not only the density of ganglion cells during disease, but also information about their morphology and an evaluation of excitatory and inhibitory synaptic inputs. Here, we describe how indirect immunofluorescence can be used in retinal whole mounts to obtain information about ganglion cells in retinal degeneration.

Original languageEnglish
Title of host publicationMouse Retinal Phenotyping
Subtitle of host publicationMethods and Protocols
EditorsNaoyuki Tanimoto
Place of PublicationUnited States
PublisherHumana Press
Chapter18
Pages261-273
Number of pages13
ISBN (Electronic)9781493977192
ISBN (Print)9781493977208
DOIs
Publication statusPublished - 2018
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1753
ISSN (Print)1064-3745

Keywords

  • Ganglion cells
  • Gephyrin
  • Indirect immunofluorescence
  • Morphology
  • Mouse
  • Retina
  • Retinal degeneration
  • RIBEYE
  • Rodent

Cite this