Gadolinium(III) spin labels for high-sensitivity distance measurements in transmembrane helices

Erez Matalon; Thomas Huber; Gregor Hagelueken; Bimbil Graham; Veronica Frydman; Akiva Feintuch; Gottfried Otting; Daniella Goldfarb

doi:10.1002/anie.201305574

Gadolinium(III) spin labels for high-sensitivity distance measurements in transmembrane helices

Erez Matalon, Thomas Huber, Gregor Hagelueken, Bimbil Graham, Veronica Frydman, Akiva Feintuch, Gottfried Otting, Daniella Goldfarb

Medicinal Chemistry

Research output: Contribution to journal › Article › Research › peer-review

44 Citations (Scopus)

Abstract

High-frequency double electron-electron resonance (DEER) distance measurements using different Gd3+ tags (Gd-DOTA and Gd-C1) were carried out on transmembrane helical peptides (ca. 0.15-nmol; WALP peptides) in a model membrane. The ability to pick up small distance variations, the chemical flexibility of the tags, and the remarkable absolute sensitivity, make this approach attractive for studies of membrane proteins. Copyright ? 2013 WILEY-VCH Verlag GmbH Co. KGaA, Weinheim.

Original language	English
Pages (from-to)	11831 - 11834
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	52
Issue number	45
DOIs	https://doi.org/10.1002/anie.201305574
Publication status	Published - 2013

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10.1002/anie.201305574

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Cite this

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title = "Gadolinium(III) spin labels for high-sensitivity distance measurements in transmembrane helices",

abstract = "High-frequency double electron-electron resonance (DEER) distance measurements using different Gd3+ tags (Gd-DOTA and Gd-C1) were carried out on transmembrane helical peptides (ca. 0.15-nmol; WALP peptides) in a model membrane. The ability to pick up small distance variations, the chemical flexibility of the tags, and the remarkable absolute sensitivity, make this approach attractive for studies of membrane proteins. Copyright ? 2013 WILEY-VCH Verlag GmbH Co. KGaA, Weinheim.",

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pages = "11831 -- 11834",

journal = "Angewandte Chemie - International Edition",

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Gadolinium(III) spin labels for high-sensitivity distance measurements in transmembrane helices. / Matalon, Erez; Huber, Thomas; Hagelueken, Gregor; Graham, Bimbil; Frydman, Veronica; Feintuch, Akiva; Otting, Gottfried; Goldfarb, Daniella.

In: Angewandte Chemie - International Edition, Vol. 52, No. 45, 2013, p. 11831 - 11834.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Gadolinium(III) spin labels for high-sensitivity distance measurements in transmembrane helices

AU - Matalon, Erez

AU - Huber, Thomas

AU - Hagelueken, Gregor

AU - Graham, Bimbil

AU - Frydman, Veronica

AU - Feintuch, Akiva

AU - Otting, Gottfried

AU - Goldfarb, Daniella

PY - 2013

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AB - High-frequency double electron-electron resonance (DEER) distance measurements using different Gd3+ tags (Gd-DOTA and Gd-C1) were carried out on transmembrane helical peptides (ca. 0.15-nmol; WALP peptides) in a model membrane. The ability to pick up small distance variations, the chemical flexibility of the tags, and the remarkable absolute sensitivity, make this approach attractive for studies of membrane proteins. Copyright ? 2013 WILEY-VCH Verlag GmbH Co. KGaA, Weinheim.

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