Gadolinium tagging for high-precision measurements of 6 nm distances in protein assemblies by EPR.

Hiromasa Yagi, Debamalya Banerjee, Bimbil Graham, Thomas Huber, Daniella Goldfarb, Gottfried Otting

Research output: Contribution to journalArticleResearchpeer-review

87 Citations (Scopus)

Abstract

Double electron-electron resonance (DEER) distance measurements of a protein complex tagged with two Gd(3+) chelates developed for rigid positioning of the metal ion are shown to deliver outstandingly accurate distance measurements in the 6 nm range. The accuracy was assessed by comparison with modeled distance distributions based on the three-dimensional molecular structures of the protein and the tag and further comparison with paramagnetic NMR data. The close agreement between the predicted and experimentally measured distances opens new possibilities for investigating the structure of biomolecular assemblies. As an example, we show that the dimer interface of rat ERp29 in solution is the same as that determined previously for human ERp29 in the single crystal.
Original languageEnglish
Pages (from-to)10418 - 10421
Number of pages4
JournalJournal of the American Chemical Society
Volume133
Issue number27
DOIs
Publication statusPublished - 2011

Cite this