G protein-coupled receptor 43 modulates neutrophil recruitment during acute inflammation

Marjon E. Kamp, Raymond Shim, Alyce J. Nicholls, Ana Carolina Oliveira, Linda J. Mason, Lauren Binge, Charles R. Mackay, Connie H. Y. Wong

Research output: Contribution to journalArticleResearchpeer-review

18 Citations (Scopus)

Abstract

Fermentation of dietary fibre in the gut yields large amounts of short chain fatty acids (SCFAs). SCFAs can impart biological responses in cells through their engagement of 'metabolite-sensing' G protein-coupled receptors (GPCRs). One of the main SCFA receptors, GPR43, is highly expressed by neutrophils, which suggests that the actions of GPR43 and dietary fibre intake may affect neutrophil recruitment during inflammatory responses in vivo. Using intravital imaging of the small intestine, we found greater intravascular neutrophil rolling and adhesion in Gpr43-/-mice in response to LPS at 1 h. After 4 h of LPS challenge, the intravascular rolling velocity of GPR43-deficient neutrophils was reduced significantly and increased numbers of neutrophils were found in the lamina propria of Gpr43-/-mice. Additionally, GPR43-deficient leukocytes demonstrated exacerbated migration into the peritoneal cavity following fMLP challenge. The fMLP-induced neutrophil migration was significantly suppressed in wildtype mice that were treated with acetate, but not in Gpr43-/-mice, strongly suggesting a role for SCFAs in modulating neutrophil migration via GPR43. Indeed, neutrophils of no fibre-fed wildtype mice exhibited elevated migratory behaviour compared to normal chow-fed wildtype mice. Interestingly, this elevated migration could also be reproduced through simple transfer of a no fibre microbiota into germ-free mice, suggesting that the composition and function of microbiota stemming from a no fibre diet mediated the changes in neutrophil migration. Therefore, GPR43 and a microbiota composition that allows for SCFA production function to modulate neutrophil recruitment during inflammatory responses.

Original languageEnglish
Article numbere0163750
Number of pages15
JournalPLoS ONE
Volume11
Issue number9
DOIs
Publication statusPublished - 22 Sep 2016

Cite this

Kamp, M. E., Shim, R., Nicholls, A. J., Oliveira, A. C., Mason, L. J., Binge, L., ... Wong, C. H. Y. (2016). G protein-coupled receptor 43 modulates neutrophil recruitment during acute inflammation. PLoS ONE, 11(9), [e0163750]. https://doi.org/10.1371/journal.pone.0163750
Kamp, Marjon E. ; Shim, Raymond ; Nicholls, Alyce J. ; Oliveira, Ana Carolina ; Mason, Linda J. ; Binge, Lauren ; Mackay, Charles R. ; Wong, Connie H. Y. / G protein-coupled receptor 43 modulates neutrophil recruitment during acute inflammation. In: PLoS ONE. 2016 ; Vol. 11, No. 9.
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G protein-coupled receptor 43 modulates neutrophil recruitment during acute inflammation. / Kamp, Marjon E.; Shim, Raymond; Nicholls, Alyce J.; Oliveira, Ana Carolina; Mason, Linda J.; Binge, Lauren; Mackay, Charles R.; Wong, Connie H. Y.

In: PLoS ONE, Vol. 11, No. 9, e0163750, 22.09.2016.

Research output: Contribution to journalArticleResearchpeer-review

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Kamp ME, Shim R, Nicholls AJ, Oliveira AC, Mason LJ, Binge L et al. G protein-coupled receptor 43 modulates neutrophil recruitment during acute inflammation. PLoS ONE. 2016 Sep 22;11(9). e0163750. https://doi.org/10.1371/journal.pone.0163750