Further limitations of phylogenetic group-specific probes used for detection of bacteria in environmental samples

Jeremy J Barr, Linda L. Blackall, Philip L. Bond

Research output: Contribution to journalComment / DebateOtherpeer-review

9 Citations (Scopus)

Abstract

Fluorescence in situ hybridization (FISH) with fluorochrome-labelled oligonucleotides targeting rRNA is a powerful tool for the identification and quantification of micro-organisms that are important in environmental and industrial processes (Amann,1995). Phylogenetic group-specific (PGS) oligonucleotide probes, targeting rRNA of many different taxa, are commonly used to screen environmental samples. The use of broad-spectrum PGS FISH probes is quite limited because they might detect micro-organisms outside the PGS group containing the target sequence (false positives), and they frequently miss micro-organisms within the group lacking the target sequence (false negatives)(Loy et al., 2007; Amann and Fuchs, 2008).
Original languageEnglish
Pages (from-to)959-961
Number of pages3
JournalISME Journal
Volume4
Issue number8
DOIs
Publication statusPublished - Aug 2010
Externally publishedYes

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