Functional identification of the cDNA coding for a wheat endo-1,4-β-D-xylanase inhibitor

Giles O. Elliott, Richard K. Hughes, Nathalie Juge, Paul A. Kroon, Gary Williamson

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41 Citations (Scopus)

Abstract

Using expressed sequence tag data, we obtained a full-length cDNA encoding a wheat protein inhibitor of xylanases (XIP-I). The 822 bp open reading frame encoded a protein of 274 amino acids with a molecular mass of 30.2 kDa, in excellent agreement with the native protein. Expression in Escherichia coli confirmed that the cDNA encoded a functional endo-1,4-β-D-xylanase inhibitor. Its deduced amino acid sequence exhibited highest similarity to sequences classified as class III chitinases, but the inhibitor did not exhibit chitinase activity. This is the first full-length cDNA sequence that encodes a novel class of protein which inhibits the activity of endo-1,4-β-D-xylanases.

Original languageEnglish
Pages (from-to)66-70
Number of pages5
JournalFEBS Letters
Volume519
Issue number1-3
DOIs
Publication statusPublished - 22 May 2002
Externally publishedYes

Keywords

  • Expressed sequence tag
  • Heterologous expression
  • Protein inhibitor
  • Triticum aestivum
  • Wheat
  • Xylanase

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