Functional antagonism between Sall4 and Plzf defines germline progenitors

Robin M Hobbs, Sharmila Fagoonee, Antonella Papa, Kaitlyn Webster, Fiorella Altruda, Ryuichi Nishinakamura, Li Chai, Pier P Pandolfi

Research output: Contribution to journalArticleResearchpeer-review

98 Citations (Scopus)

Abstract

Transcription factors required for formation of embryonic tissues often maintain their expression in adult stem cell populations, but whether their function remains equivalent is not clear. Here we demonstrate critical and distinct roles for Sall4 in development of embryonic germ cells and differentiation of postnatal spermatogonial progenitor cells (SPCs). In differentiating SPCs, Sall4 levels transiently increase and Sall4 physically interacts with Plzf, a transcription factor exclusively required for adult stem cell maintenance. Mechanistically, Sall4 sequesters Plzf to noncognate chromatin domains to induce expression of Kit, a target of Plzf-mediated repression required for differentiation. Plzf in turn antagonizes Sall4 function by displacing Sall4 from cognate chromatin to induce Sall1 expression. Taken together, these data suggest that transcription factors required for embryonic tissue development postnatally take on distinct roles through interaction with opposing factors, which hence define properties of the adult stem cell compartment.
Original languageEnglish
Pages (from-to)284 - 298
Number of pages15
JournalCell Stem Cell
Volume10
Issue number3
DOIs
Publication statusPublished - 2012

Cite this

Hobbs, R. M., Fagoonee, S., Papa, A., Webster, K., Altruda, F., Nishinakamura, R., ... Pandolfi, P. P. (2012). Functional antagonism between Sall4 and Plzf defines germline progenitors. Cell Stem Cell, 10(3), 284 - 298. https://doi.org/10.1016/j.stem.2012.02.004
Hobbs, Robin M ; Fagoonee, Sharmila ; Papa, Antonella ; Webster, Kaitlyn ; Altruda, Fiorella ; Nishinakamura, Ryuichi ; Chai, Li ; Pandolfi, Pier P. / Functional antagonism between Sall4 and Plzf defines germline progenitors. In: Cell Stem Cell. 2012 ; Vol. 10, No. 3. pp. 284 - 298.
@article{c9a02671d863490e98bba5dc4bbade41,
title = "Functional antagonism between Sall4 and Plzf defines germline progenitors",
abstract = "Transcription factors required for formation of embryonic tissues often maintain their expression in adult stem cell populations, but whether their function remains equivalent is not clear. Here we demonstrate critical and distinct roles for Sall4 in development of embryonic germ cells and differentiation of postnatal spermatogonial progenitor cells (SPCs). In differentiating SPCs, Sall4 levels transiently increase and Sall4 physically interacts with Plzf, a transcription factor exclusively required for adult stem cell maintenance. Mechanistically, Sall4 sequesters Plzf to noncognate chromatin domains to induce expression of Kit, a target of Plzf-mediated repression required for differentiation. Plzf in turn antagonizes Sall4 function by displacing Sall4 from cognate chromatin to induce Sall1 expression. Taken together, these data suggest that transcription factors required for embryonic tissue development postnatally take on distinct roles through interaction with opposing factors, which hence define properties of the adult stem cell compartment.",
author = "Hobbs, {Robin M} and Sharmila Fagoonee and Antonella Papa and Kaitlyn Webster and Fiorella Altruda and Ryuichi Nishinakamura and Li Chai and Pandolfi, {Pier P}",
year = "2012",
doi = "10.1016/j.stem.2012.02.004",
language = "English",
volume = "10",
pages = "284 -- 298",
journal = "Cell Stem Cell",
issn = "1934-5909",
publisher = "Elsevier",
number = "3",

}

Hobbs, RM, Fagoonee, S, Papa, A, Webster, K, Altruda, F, Nishinakamura, R, Chai, L & Pandolfi, PP 2012, 'Functional antagonism between Sall4 and Plzf defines germline progenitors', Cell Stem Cell, vol. 10, no. 3, pp. 284 - 298. https://doi.org/10.1016/j.stem.2012.02.004

Functional antagonism between Sall4 and Plzf defines germline progenitors. / Hobbs, Robin M; Fagoonee, Sharmila; Papa, Antonella; Webster, Kaitlyn; Altruda, Fiorella; Nishinakamura, Ryuichi; Chai, Li; Pandolfi, Pier P.

In: Cell Stem Cell, Vol. 10, No. 3, 2012, p. 284 - 298.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Functional antagonism between Sall4 and Plzf defines germline progenitors

AU - Hobbs, Robin M

AU - Fagoonee, Sharmila

AU - Papa, Antonella

AU - Webster, Kaitlyn

AU - Altruda, Fiorella

AU - Nishinakamura, Ryuichi

AU - Chai, Li

AU - Pandolfi, Pier P

PY - 2012

Y1 - 2012

N2 - Transcription factors required for formation of embryonic tissues often maintain their expression in adult stem cell populations, but whether their function remains equivalent is not clear. Here we demonstrate critical and distinct roles for Sall4 in development of embryonic germ cells and differentiation of postnatal spermatogonial progenitor cells (SPCs). In differentiating SPCs, Sall4 levels transiently increase and Sall4 physically interacts with Plzf, a transcription factor exclusively required for adult stem cell maintenance. Mechanistically, Sall4 sequesters Plzf to noncognate chromatin domains to induce expression of Kit, a target of Plzf-mediated repression required for differentiation. Plzf in turn antagonizes Sall4 function by displacing Sall4 from cognate chromatin to induce Sall1 expression. Taken together, these data suggest that transcription factors required for embryonic tissue development postnatally take on distinct roles through interaction with opposing factors, which hence define properties of the adult stem cell compartment.

AB - Transcription factors required for formation of embryonic tissues often maintain their expression in adult stem cell populations, but whether their function remains equivalent is not clear. Here we demonstrate critical and distinct roles for Sall4 in development of embryonic germ cells and differentiation of postnatal spermatogonial progenitor cells (SPCs). In differentiating SPCs, Sall4 levels transiently increase and Sall4 physically interacts with Plzf, a transcription factor exclusively required for adult stem cell maintenance. Mechanistically, Sall4 sequesters Plzf to noncognate chromatin domains to induce expression of Kit, a target of Plzf-mediated repression required for differentiation. Plzf in turn antagonizes Sall4 function by displacing Sall4 from cognate chromatin to induce Sall1 expression. Taken together, these data suggest that transcription factors required for embryonic tissue development postnatally take on distinct roles through interaction with opposing factors, which hence define properties of the adult stem cell compartment.

UR - http://www.sciencedirect.com/science/article/pii/S1934590912000628

U2 - 10.1016/j.stem.2012.02.004

DO - 10.1016/j.stem.2012.02.004

M3 - Article

VL - 10

SP - 284

EP - 298

JO - Cell Stem Cell

JF - Cell Stem Cell

SN - 1934-5909

IS - 3

ER -