Function of the rat calcitonin receptors, C1a and C1b, expressed in Xenopus oocytes

Masanori Matsumoto, Muneshige Kaibara, Yasuhito Uezono, Futoshi Izumi, Koji Sumikawa, Patrick M. Sexton, Kohtaro Taniyama

Research output: Contribution to journalArticleResearchpeer-review

7 Citations (Scopus)

Abstract

The function of the cloned rat calcitonin receptors, C1a and C1b, was studied in Xenopus oocytes using the two-electrode voltage clamp method. In oocytes expressing the C1a receptors and the cystic fibrosis transmembrane conductance regulator (CFTR), C1a/ CFTR, application (30 sec) of either salmon calcitonin (sCT) or human calcitonin (hCT) activated currents through CFTR. In C1b/CFTR, sCT activated the currents, whereas hCT failed to elicit a response. The sCT induced currents in C1a/CFTR were similar in size to those in C1b/CFTR. Both the activation and the deactivation of sCT-induced currents were slower in C1a/ CFTR. In oocytes expressing C1a or C1b alone, application of relatively high concentrations of sCT induced small oscillatory inward currents. Application of hCT induced small inward currents in C1a alone, but failed to activate currents in C1b alone. These results demonstrate new insights into the signal transduction of calcitonin receptors.

Original languageEnglish
Pages (from-to)484-491
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume242
Issue number3
DOIs
Publication statusPublished - 26 Jan 1998
Externally publishedYes

Cite this

Matsumoto, Masanori ; Kaibara, Muneshige ; Uezono, Yasuhito ; Izumi, Futoshi ; Sumikawa, Koji ; Sexton, Patrick M. ; Taniyama, Kohtaro. / Function of the rat calcitonin receptors, C1a and C1b, expressed in Xenopus oocytes. In: Biochemical and Biophysical Research Communications. 1998 ; Vol. 242, No. 3. pp. 484-491.
@article{4b9aeaeabcee4a0698cc889ccc1dcdf1,
title = "Function of the rat calcitonin receptors, C1a and C1b, expressed in Xenopus oocytes",
abstract = "The function of the cloned rat calcitonin receptors, C1a and C1b, was studied in Xenopus oocytes using the two-electrode voltage clamp method. In oocytes expressing the C1a receptors and the cystic fibrosis transmembrane conductance regulator (CFTR), C1a/ CFTR, application (30 sec) of either salmon calcitonin (sCT) or human calcitonin (hCT) activated currents through CFTR. In C1b/CFTR, sCT activated the currents, whereas hCT failed to elicit a response. The sCT induced currents in C1a/CFTR were similar in size to those in C1b/CFTR. Both the activation and the deactivation of sCT-induced currents were slower in C1a/ CFTR. In oocytes expressing C1a or C1b alone, application of relatively high concentrations of sCT induced small oscillatory inward currents. Application of hCT induced small inward currents in C1a alone, but failed to activate currents in C1b alone. These results demonstrate new insights into the signal transduction of calcitonin receptors.",
author = "Masanori Matsumoto and Muneshige Kaibara and Yasuhito Uezono and Futoshi Izumi and Koji Sumikawa and Sexton, {Patrick M.} and Kohtaro Taniyama",
year = "1998",
month = "1",
day = "26",
doi = "10.1006/bbrc.1997.7991",
language = "English",
volume = "242",
pages = "484--491",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier",
number = "3",

}

Function of the rat calcitonin receptors, C1a and C1b, expressed in Xenopus oocytes. / Matsumoto, Masanori; Kaibara, Muneshige; Uezono, Yasuhito; Izumi, Futoshi; Sumikawa, Koji; Sexton, Patrick M.; Taniyama, Kohtaro.

In: Biochemical and Biophysical Research Communications, Vol. 242, No. 3, 26.01.1998, p. 484-491.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Function of the rat calcitonin receptors, C1a and C1b, expressed in Xenopus oocytes

AU - Matsumoto, Masanori

AU - Kaibara, Muneshige

AU - Uezono, Yasuhito

AU - Izumi, Futoshi

AU - Sumikawa, Koji

AU - Sexton, Patrick M.

AU - Taniyama, Kohtaro

PY - 1998/1/26

Y1 - 1998/1/26

N2 - The function of the cloned rat calcitonin receptors, C1a and C1b, was studied in Xenopus oocytes using the two-electrode voltage clamp method. In oocytes expressing the C1a receptors and the cystic fibrosis transmembrane conductance regulator (CFTR), C1a/ CFTR, application (30 sec) of either salmon calcitonin (sCT) or human calcitonin (hCT) activated currents through CFTR. In C1b/CFTR, sCT activated the currents, whereas hCT failed to elicit a response. The sCT induced currents in C1a/CFTR were similar in size to those in C1b/CFTR. Both the activation and the deactivation of sCT-induced currents were slower in C1a/ CFTR. In oocytes expressing C1a or C1b alone, application of relatively high concentrations of sCT induced small oscillatory inward currents. Application of hCT induced small inward currents in C1a alone, but failed to activate currents in C1b alone. These results demonstrate new insights into the signal transduction of calcitonin receptors.

AB - The function of the cloned rat calcitonin receptors, C1a and C1b, was studied in Xenopus oocytes using the two-electrode voltage clamp method. In oocytes expressing the C1a receptors and the cystic fibrosis transmembrane conductance regulator (CFTR), C1a/ CFTR, application (30 sec) of either salmon calcitonin (sCT) or human calcitonin (hCT) activated currents through CFTR. In C1b/CFTR, sCT activated the currents, whereas hCT failed to elicit a response. The sCT induced currents in C1a/CFTR were similar in size to those in C1b/CFTR. Both the activation and the deactivation of sCT-induced currents were slower in C1a/ CFTR. In oocytes expressing C1a or C1b alone, application of relatively high concentrations of sCT induced small oscillatory inward currents. Application of hCT induced small inward currents in C1a alone, but failed to activate currents in C1b alone. These results demonstrate new insights into the signal transduction of calcitonin receptors.

UR - http://www.scopus.com/inward/record.url?scp=0032567677&partnerID=8YFLogxK

U2 - 10.1006/bbrc.1997.7991

DO - 10.1006/bbrc.1997.7991

M3 - Article

C2 - 9464242

AN - SCOPUS:0032567677

VL - 242

SP - 484

EP - 491

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 3

ER -