FT-IR imaging for quantitative determination of liver fat content in non-alcoholic fatty liver

K. Kochan, E. Maslak, S. Chlopicki, M. Baranska

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13 Citations (Scopus)

Abstract

In this work we apply FT-IR imaging of large areas of liver tissue cross-section samples (∼5 cm × 5 cm) for quantitative assessment of steatosis in murine model of Non-Alcoholic Fatty Liver (NAFLD). We quantified the area of liver tissue occupied by lipid droplets (LDs) by FT-IR imaging and Oil Red O (ORO) staining for comparison. Two alternative FT-IR based approaches are presented. The first, straightforward method, was based on average spectra from tissues and provided values of the fat content by using a PLS regression model and the reference method. The second one – the chemometric-based method – enabled us to determine the values of the fat content, independently of the reference method by means of k-means cluster (KMC) analysis. In summary, FT-IR images of large size liver sections may prove to be useful for quantifying liver steatosis without the need of tissue staining.

Original languageEnglish
Pages (from-to)4997-5002
Number of pages6
JournalAnalyst
Volume140
Issue number15
DOIs
Publication statusPublished - 18 May 2015
Externally publishedYes

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