FT-IR- and Raman-based biochemical profiling of the early stage of pulmonary metastasis of breast cancer in mice

Karolina Chrabaszcz, Kamila Kochan, Andrzej Fedorowicz, Agnieszka Jasztal, Elzbieta Buczek, Lisa S. Leslie, Rohit Bhargava, Kamilla Malek, Stefan Chlopicki, Katarzyna M. Marzec

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The combination of FT-IR and Raman spectroscopies allowed the biochemical profiling of lungs in the early stage of pulmonary metastasis in the murine model of breast cancer. Histological staining was used as a reference. Raman spectroscopy was especially useful in the detection and semi-quantitative analysis of the vitamin A content in lung lipofibroblasts, whereas the IR technique provided semi-quantitative information on the contents of nucleic acids, carbohydrates including glycogen, and lipids as well as changes in the secondary structures of tissue proteins. Our spectroscopic results suggest that the early phase of metastasis in the lung is characterized by a decrease in the endogenous retinoid content in combination with a decrease in the content of glycogen and lipids.

Original languageEnglish
Pages (from-to)2042-2050
Number of pages9
JournalAnalyst
Volume143
Issue number9
DOIs
Publication statusPublished - 7 May 2018

Cite this

Chrabaszcz, K., Kochan, K., Fedorowicz, A., Jasztal, A., Buczek, E., Leslie, L. S., ... Marzec, K. M. (2018). FT-IR- and Raman-based biochemical profiling of the early stage of pulmonary metastasis of breast cancer in mice. Analyst, 143(9), 2042-2050. https://doi.org/10.1039/c7an01883e
Chrabaszcz, Karolina ; Kochan, Kamila ; Fedorowicz, Andrzej ; Jasztal, Agnieszka ; Buczek, Elzbieta ; Leslie, Lisa S. ; Bhargava, Rohit ; Malek, Kamilla ; Chlopicki, Stefan ; Marzec, Katarzyna M. / FT-IR- and Raman-based biochemical profiling of the early stage of pulmonary metastasis of breast cancer in mice. In: Analyst. 2018 ; Vol. 143, No. 9. pp. 2042-2050.
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title = "FT-IR- and Raman-based biochemical profiling of the early stage of pulmonary metastasis of breast cancer in mice",
abstract = "The combination of FT-IR and Raman spectroscopies allowed the biochemical profiling of lungs in the early stage of pulmonary metastasis in the murine model of breast cancer. Histological staining was used as a reference. Raman spectroscopy was especially useful in the detection and semi-quantitative analysis of the vitamin A content in lung lipofibroblasts, whereas the IR technique provided semi-quantitative information on the contents of nucleic acids, carbohydrates including glycogen, and lipids as well as changes in the secondary structures of tissue proteins. Our spectroscopic results suggest that the early phase of metastasis in the lung is characterized by a decrease in the endogenous retinoid content in combination with a decrease in the content of glycogen and lipids.",
author = "Karolina Chrabaszcz and Kamila Kochan and Andrzej Fedorowicz and Agnieszka Jasztal and Elzbieta Buczek and Leslie, {Lisa S.} and Rohit Bhargava and Kamilla Malek and Stefan Chlopicki and Marzec, {Katarzyna M.}",
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Chrabaszcz, K, Kochan, K, Fedorowicz, A, Jasztal, A, Buczek, E, Leslie, LS, Bhargava, R, Malek, K, Chlopicki, S & Marzec, KM 2018, 'FT-IR- and Raman-based biochemical profiling of the early stage of pulmonary metastasis of breast cancer in mice', Analyst, vol. 143, no. 9, pp. 2042-2050. https://doi.org/10.1039/c7an01883e

FT-IR- and Raman-based biochemical profiling of the early stage of pulmonary metastasis of breast cancer in mice. / Chrabaszcz, Karolina; Kochan, Kamila; Fedorowicz, Andrzej; Jasztal, Agnieszka; Buczek, Elzbieta; Leslie, Lisa S.; Bhargava, Rohit; Malek, Kamilla; Chlopicki, Stefan; Marzec, Katarzyna M.

In: Analyst, Vol. 143, No. 9, 07.05.2018, p. 2042-2050.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - FT-IR- and Raman-based biochemical profiling of the early stage of pulmonary metastasis of breast cancer in mice

AU - Chrabaszcz, Karolina

AU - Kochan, Kamila

AU - Fedorowicz, Andrzej

AU - Jasztal, Agnieszka

AU - Buczek, Elzbieta

AU - Leslie, Lisa S.

AU - Bhargava, Rohit

AU - Malek, Kamilla

AU - Chlopicki, Stefan

AU - Marzec, Katarzyna M.

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Y1 - 2018/5/7

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