TY - JOUR
T1 - Frozen-thawed epididymal spermatozoa for intracytoplasmic sperm injection
AU - Holden, Carol A.
AU - Fuscaldo, Giuliana F.
AU - Jackson, Peter
AU - Cato, Alex
AU - Southwick, Graeme J.
AU - Hauser, Ron
AU - Temple-Smith, Peter D.
AU - McLachlan, Robert I.
PY - 1997
Y1 - 1997
N2 - Objective: To report the fertilization rate and pregnancy results for intracytoplasmic sperm injection (ICSI) cycles using frozen-thawed epididymal spermatozoa. Design: Retrospective analysis of consecutive ICSI cycles. Setting: Tertiary referral center for infertility. Patient(s): Infertile couples in whom 39 patients (59 ICSI cycles) required the use of frozen- thawed epididymal spermatozoa. As no cycles were performed using fresh epididymal spermatozoa, outcomes were compared with those of 130 couples (170 ICSI cycles) using fresh ejaculated spermatozoa for severe oligoasthenozoospermia-teratozoospermia, in which 20%, compared with lower vitality, both the fertilization (56% versus 22%) and embryo cleavage (91% versus 57%) rates were significantly greater. Conclusion(s): The routine collection and cryopreservation of epididymal spermatozoa at microsurgery allows multiple ICSI treatment cycles with success rates similar to those of ejaculated spermatozoa. However, when the vitality of aspirated spermatozoa is
AB - Objective: To report the fertilization rate and pregnancy results for intracytoplasmic sperm injection (ICSI) cycles using frozen-thawed epididymal spermatozoa. Design: Retrospective analysis of consecutive ICSI cycles. Setting: Tertiary referral center for infertility. Patient(s): Infertile couples in whom 39 patients (59 ICSI cycles) required the use of frozen- thawed epididymal spermatozoa. As no cycles were performed using fresh epididymal spermatozoa, outcomes were compared with those of 130 couples (170 ICSI cycles) using fresh ejaculated spermatozoa for severe oligoasthenozoospermia-teratozoospermia, in which 20%, compared with lower vitality, both the fertilization (56% versus 22%) and embryo cleavage (91% versus 57%) rates were significantly greater. Conclusion(s): The routine collection and cryopreservation of epididymal spermatozoa at microsurgery allows multiple ICSI treatment cycles with success rates similar to those of ejaculated spermatozoa. However, when the vitality of aspirated spermatozoa is
KW - cryopreservation
KW - Epididymal sperm
KW - ICSI
UR - http://www.scopus.com/inward/record.url?scp=0031018347&partnerID=8YFLogxK
U2 - 10.1016/S0015-0282(97)81860-2
DO - 10.1016/S0015-0282(97)81860-2
M3 - Article
C2 - 8986688
AN - SCOPUS:0031018347
VL - 67
SP - 81
EP - 87
JO - Fertility and Sterility
JF - Fertility and Sterility
SN - 0015-0282
IS - 1
ER -