TY - JOUR
T1 - From whole-mount to single-cell spatial assessment of gene expression in 3D
AU - Waylen, Lisa N.
AU - Nim, Hieu T.
AU - Martelotto, Luciano G.
AU - Ramialison, Mirana
PY - 2020/10/23
Y1 - 2020/10/23
N2 - Unravelling spatio-temporal patterns of gene expression is crucial to understanding core biological principles from embryogenesis to disease. Here we review emerging technologies, providing automated, high-throughput, spatially resolved quantitative gene expression data. Novel techniques expand on current benchmark protocols, expediting their incorporation into ongoing research. These approaches digitally reconstruct patterns of embryonic expression in three dimensions, and have successfully identified novel domains of expression, cell types, and tissue features. Such technologies pave the way for unbiased and exhaustive recapitulation of gene expression levels in spatial and quantitative terms, promoting understanding of the molecular origin of developmental defects, and improving medical diagnostics.
AB - Unravelling spatio-temporal patterns of gene expression is crucial to understanding core biological principles from embryogenesis to disease. Here we review emerging technologies, providing automated, high-throughput, spatially resolved quantitative gene expression data. Novel techniques expand on current benchmark protocols, expediting their incorporation into ongoing research. These approaches digitally reconstruct patterns of embryonic expression in three dimensions, and have successfully identified novel domains of expression, cell types, and tissue features. Such technologies pave the way for unbiased and exhaustive recapitulation of gene expression levels in spatial and quantitative terms, promoting understanding of the molecular origin of developmental defects, and improving medical diagnostics.
KW - data acquisition
KW - transcriptomics
UR - http://www.scopus.com/inward/record.url?scp=85093929217&partnerID=8YFLogxK
U2 - 10.1038/s42003-020-01341-1
DO - 10.1038/s42003-020-01341-1
M3 - Review Article
C2 - 33097816
AN - SCOPUS:85093929217
VL - 3
JO - Communications Biology
JF - Communications Biology
SN - 2399-3642
IS - 1
M1 - 602
ER -