Abstract
The BH3-only protein Bim is a critical initiator of apoptosis in hematopoietic cells. Bim is upregulated in response to growth factor withdrawal and in vitro studies have implicated the transcription factor Foxo3a as a critical inducer. To test the importance of this regulation in vivo, we generated mice with mutated Foxo-binding sites within the Bim promoters (Bim?Foxo/?Foxo). Contrary to Bim-deficient mice, Bim?Foxo/?Foxo mice had a normal hematopoietic system. Moreover, cytokine-dependent haematopoietic cells from Bim ?Foxo/?Foxo and wt mice died at similar rates. These results indicate that regulation of Bim by Foxo transcription factors is not critical for the killing of hematopoietic cells. ? 2013 European Molecular Biology Organization
Original language | English |
---|---|
Pages (from-to) | 992 - 998 |
Number of pages | 7 |
Journal | EMBO Reports |
Volume | 14 |
Issue number | 11 |
DOIs | |
Publication status | Published - 2013 |