Foxo-mediated Bim transcription is dispensable for the apoptosis of hematopoietic cells that is mediated by this BH3-only protein

Marco Herold, Leona Rohrbeck, Mathias Lang, Raelene Joy Grumont, Steven Demetrious Gerondakis, Lin Tai, Phillipe Bouillet, Thomas Kaufmann, Andreas Strasser

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The BH3-only protein Bim is a critical initiator of apoptosis in hematopoietic cells. Bim is upregulated in response to growth factor withdrawal and in vitro studies have implicated the transcription factor Foxo3a as a critical inducer. To test the importance of this regulation in vivo, we generated mice with mutated Foxo-binding sites within the Bim promoters (Bim?Foxo/?Foxo). Contrary to Bim-deficient mice, Bim?Foxo/?Foxo mice had a normal hematopoietic system. Moreover, cytokine-dependent haematopoietic cells from Bim ?Foxo/?Foxo and wt mice died at similar rates. These results indicate that regulation of Bim by Foxo transcription factors is not critical for the killing of hematopoietic cells. ? 2013 European Molecular Biology Organization
Original languageEnglish
Pages (from-to)992 - 998
Number of pages7
JournalEMBO Reports
Volume14
Issue number11
DOIs
Publication statusPublished - 2013

Cite this

Herold, Marco ; Rohrbeck, Leona ; Lang, Mathias ; Grumont, Raelene Joy ; Gerondakis, Steven Demetrious ; Tai, Lin ; Bouillet, Phillipe ; Kaufmann, Thomas ; Strasser, Andreas. / Foxo-mediated Bim transcription is dispensable for the apoptosis of hematopoietic cells that is mediated by this BH3-only protein. In: EMBO Reports. 2013 ; Vol. 14, No. 11. pp. 992 - 998.
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abstract = "The BH3-only protein Bim is a critical initiator of apoptosis in hematopoietic cells. Bim is upregulated in response to growth factor withdrawal and in vitro studies have implicated the transcription factor Foxo3a as a critical inducer. To test the importance of this regulation in vivo, we generated mice with mutated Foxo-binding sites within the Bim promoters (Bim?Foxo/?Foxo). Contrary to Bim-deficient mice, Bim?Foxo/?Foxo mice had a normal hematopoietic system. Moreover, cytokine-dependent haematopoietic cells from Bim ?Foxo/?Foxo and wt mice died at similar rates. These results indicate that regulation of Bim by Foxo transcription factors is not critical for the killing of hematopoietic cells. ? 2013 European Molecular Biology Organization",
author = "Marco Herold and Leona Rohrbeck and Mathias Lang and Grumont, {Raelene Joy} and Gerondakis, {Steven Demetrious} and Lin Tai and Phillipe Bouillet and Thomas Kaufmann and Andreas Strasser",
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Herold, M, Rohrbeck, L, Lang, M, Grumont, RJ, Gerondakis, SD, Tai, L, Bouillet, P, Kaufmann, T & Strasser, A 2013, 'Foxo-mediated Bim transcription is dispensable for the apoptosis of hematopoietic cells that is mediated by this BH3-only protein' EMBO Reports, vol. 14, no. 11, pp. 992 - 998. https://doi.org/10.1038/embor.2013.152

Foxo-mediated Bim transcription is dispensable for the apoptosis of hematopoietic cells that is mediated by this BH3-only protein. / Herold, Marco; Rohrbeck, Leona; Lang, Mathias; Grumont, Raelene Joy; Gerondakis, Steven Demetrious; Tai, Lin; Bouillet, Phillipe; Kaufmann, Thomas; Strasser, Andreas.

In: EMBO Reports, Vol. 14, No. 11, 2013, p. 992 - 998.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Herold, Marco

AU - Rohrbeck, Leona

AU - Lang, Mathias

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AU - Gerondakis, Steven Demetrious

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AU - Strasser, Andreas

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AB - The BH3-only protein Bim is a critical initiator of apoptosis in hematopoietic cells. Bim is upregulated in response to growth factor withdrawal and in vitro studies have implicated the transcription factor Foxo3a as a critical inducer. To test the importance of this regulation in vivo, we generated mice with mutated Foxo-binding sites within the Bim promoters (Bim?Foxo/?Foxo). Contrary to Bim-deficient mice, Bim?Foxo/?Foxo mice had a normal hematopoietic system. Moreover, cytokine-dependent haematopoietic cells from Bim ?Foxo/?Foxo and wt mice died at similar rates. These results indicate that regulation of Bim by Foxo transcription factors is not critical for the killing of hematopoietic cells. ? 2013 European Molecular Biology Organization

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