Fourier transform infrared microspectroscopy reveals unique phenotypes for human embryonic and induced pluripotent stem cell lines and their progeny

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Abstract

Fourier transform infrared (FTIR) microspectroscopy was employed to elucidate the macromolecular phenotype of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) and their differentiated progeny. Undifferentiated hESCs and hiPSC lines were found to be not clearly distinguishable from each other. However, although both hESC and hiPSC variants appeared to undergo similar changes during differentiation in terms of cell surface antigens, the derived cell types from all cell lines could be discriminated using FTIR spectroscopy. We foresee a possible future role for FTIR microspectroscopy as a powerful and objective investigative and quality control tool in regenerative medicine. ((c) 2013 WILEY-VCH Verlag GmbH Co. KGaA, Weinheim).
Original languageEnglish
Pages (from-to)767 - 781
Number of pages15
JournalJournal of Biophotonics
Volume7
Issue number10
DOIs
Publication statusPublished - 2014

Cite this

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title = "Fourier transform infrared microspectroscopy reveals unique phenotypes for human embryonic and induced pluripotent stem cell lines and their progeny",
abstract = "Fourier transform infrared (FTIR) microspectroscopy was employed to elucidate the macromolecular phenotype of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) and their differentiated progeny. Undifferentiated hESCs and hiPSC lines were found to be not clearly distinguishable from each other. However, although both hESC and hiPSC variants appeared to undergo similar changes during differentiation in terms of cell surface antigens, the derived cell types from all cell lines could be discriminated using FTIR spectroscopy. We foresee a possible future role for FTIR microspectroscopy as a powerful and objective investigative and quality control tool in regenerative medicine. ((c) 2013 WILEY-VCH Verlag GmbH Co. KGaA, Weinheim).",
author = "Julie Cao and Ng, {Elizabeth S S} and Donald McNaughton and Edouard Stanley and Elefanty, {Andrew George} and Tobin, {Mark J} and Heraud, {Philip Robert}",
year = "2014",
doi = "10.1002/jbio.201200217",
language = "English",
volume = "7",
pages = "767 -- 781",
journal = "Journal of Biophotonics",
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publisher = "Wiley-VCH Verlag GmbH & Co. KGaA",
number = "10",

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TY - JOUR

T1 - Fourier transform infrared microspectroscopy reveals unique phenotypes for human embryonic and induced pluripotent stem cell lines and their progeny

AU - Cao, Julie

AU - Ng, Elizabeth S S

AU - McNaughton, Donald

AU - Stanley, Edouard

AU - Elefanty, Andrew George

AU - Tobin, Mark J

AU - Heraud, Philip Robert

PY - 2014

Y1 - 2014

N2 - Fourier transform infrared (FTIR) microspectroscopy was employed to elucidate the macromolecular phenotype of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) and their differentiated progeny. Undifferentiated hESCs and hiPSC lines were found to be not clearly distinguishable from each other. However, although both hESC and hiPSC variants appeared to undergo similar changes during differentiation in terms of cell surface antigens, the derived cell types from all cell lines could be discriminated using FTIR spectroscopy. We foresee a possible future role for FTIR microspectroscopy as a powerful and objective investigative and quality control tool in regenerative medicine. ((c) 2013 WILEY-VCH Verlag GmbH Co. KGaA, Weinheim).

AB - Fourier transform infrared (FTIR) microspectroscopy was employed to elucidate the macromolecular phenotype of human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) and their differentiated progeny. Undifferentiated hESCs and hiPSC lines were found to be not clearly distinguishable from each other. However, although both hESC and hiPSC variants appeared to undergo similar changes during differentiation in terms of cell surface antigens, the derived cell types from all cell lines could be discriminated using FTIR spectroscopy. We foresee a possible future role for FTIR microspectroscopy as a powerful and objective investigative and quality control tool in regenerative medicine. ((c) 2013 WILEY-VCH Verlag GmbH Co. KGaA, Weinheim).

UR - http://onlinelibrary.wiley.com/doi/10.1002/jbio.201200217/pdf

U2 - 10.1002/jbio.201200217

DO - 10.1002/jbio.201200217

M3 - Article

VL - 7

SP - 767

EP - 781

JO - Journal of Biophotonics

JF - Journal of Biophotonics

SN - 1864-063X

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