Fourier transform infrared microspectroscopy identifies early lineage commitment in differentiating human embryonic stem cells

Philip Robert Heraud, Elizabeth Siew Sun Ng, Sally Caine, Qing Cissy Yu, Claire Elizabeth Hirst, Robyn Anne Mayberry, Amanda Jayne Bruce, Bayden Robert Wood, Donald McNaughton, Edouard Stanley, Andrew George Elefanty

Research output: Contribution to journalArticleResearchpeer-review

51 Citations (Scopus)

Abstract

Human ESCs (hESCs) are a valuable tool for the study of early human development and represent a source of normal differentiated cells for pharmaceutical and biotechnology applications and ultimately for cell replacement therapies. For all applications, it will be necessary to develop assays to validate the efficacy of hESC differentiation. We explored the capacity for FTIR spectroscopy, a technique that rapidly characterises cellular macromolecular composition, to discriminate mesendoderm or ectoderm committed cells from undifferentiated hESCs. Distinct infrared spectroscopic a??signaturesa?? readily distinguished hESCs from these early differentiated progeny, with bioinformatic models able to correctly classify over 97 of spectra. These data identify a role for FTIR spectroscopy as a new modality to complement conventional analyses of hESCs and their derivatives. FTIR spectroscopy has the potential to provide low-cost, automatable measurements for the quality control of stem and differentiated cells to be used in industry and regenerative medicine.
Original languageEnglish
Pages (from-to)140 - 147
Number of pages8
JournalStem Cell Research
Volume4
Issue number2
DOIs
Publication statusPublished - 2010

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