Fourier transform infrared microspectroscopy as a tool to identify macroalgal propagules

Alecia Bellgrove, Hiroshi Kihara, Akira Iwata, Masakazu N Aoki, Philip Robert Heraud

    Research output: Contribution to journalArticleResearchpeer-review

    Abstract

    Understanding of macroalgal dispersal has been hindered by the difficulty in identifying propagules. Different carrageenans typically occur in gametophytes and tetrasporophytes of the red algal family Gigartinaceae, and we may expect that carpospores and tetraspores also differ in composition of carrageenans. Using Fourier transform infrared (FT-IR) microspectroscopy, we tested the model that differences in carrageenans and other cellular constituents between nuclear phases should allow us to discriminate carpospores and tetraspores of Chondrus verrucosus Mikami. Spectral data suggest that carposporophytes isolated from the pericarp and female gametophytes contained I?-carrageenan, whereas tetrasporophytes contained I>-carrageenan. However, both carpospores and tetraspores exhibited absorbances in wave bands characteristic of I?-, I?-, and I>-carrageenans. Carpospores contained more proteins and may be more photosynthetically active than tetraspores, which contained more lipid reserves. We draw analogies to planktotrophic and lecithotrophic larvae. These differences in cellular chemistry allowed reliable discrimination of spores, but pretreatment of spectral data affected the accuracy of classification. The best classification of spores was achieved with extended multiplicative signal correction (EMSC) pretreatment using partial least squares discrimination analysis, with correct classification of 86 of carpospores and 83 of tetraspores. Classification may be further improved by using synchrotron FT-IR microspectroscopy because of its inherently higher signal-to-noise ratio compared with microspectroscopy using conventional sources of IR. This study demonstrates that FT-IR microspectroscopy and bioinformatics are useful tools to advance our understanding of algal dispersal ecology through discrimination of morphologically similar propagules both within and potentially between species.
    Original languageEnglish
    Pages (from-to)560 - 570
    Number of pages11
    JournalJournal of Phycology
    Volume45
    Issue number3
    Publication statusPublished - 2009

    Cite this

    Bellgrove, Alecia ; Kihara, Hiroshi ; Iwata, Akira ; Aoki, Masakazu N ; Heraud, Philip Robert. / Fourier transform infrared microspectroscopy as a tool to identify macroalgal propagules. In: Journal of Phycology. 2009 ; Vol. 45, No. 3. pp. 560 - 570.
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    abstract = "Understanding of macroalgal dispersal has been hindered by the difficulty in identifying propagules. Different carrageenans typically occur in gametophytes and tetrasporophytes of the red algal family Gigartinaceae, and we may expect that carpospores and tetraspores also differ in composition of carrageenans. Using Fourier transform infrared (FT-IR) microspectroscopy, we tested the model that differences in carrageenans and other cellular constituents between nuclear phases should allow us to discriminate carpospores and tetraspores of Chondrus verrucosus Mikami. Spectral data suggest that carposporophytes isolated from the pericarp and female gametophytes contained I?-carrageenan, whereas tetrasporophytes contained I>-carrageenan. However, both carpospores and tetraspores exhibited absorbances in wave bands characteristic of I?-, I?-, and I>-carrageenans. Carpospores contained more proteins and may be more photosynthetically active than tetraspores, which contained more lipid reserves. We draw analogies to planktotrophic and lecithotrophic larvae. These differences in cellular chemistry allowed reliable discrimination of spores, but pretreatment of spectral data affected the accuracy of classification. The best classification of spores was achieved with extended multiplicative signal correction (EMSC) pretreatment using partial least squares discrimination analysis, with correct classification of 86 of carpospores and 83 of tetraspores. Classification may be further improved by using synchrotron FT-IR microspectroscopy because of its inherently higher signal-to-noise ratio compared with microspectroscopy using conventional sources of IR. This study demonstrates that FT-IR microspectroscopy and bioinformatics are useful tools to advance our understanding of algal dispersal ecology through discrimination of morphologically similar propagules both within and potentially between species.",
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    Bellgrove, A, Kihara, H, Iwata, A, Aoki, MN & Heraud, PR 2009, 'Fourier transform infrared microspectroscopy as a tool to identify macroalgal propagules' Journal of Phycology, vol. 45, no. 3, pp. 560 - 570.

    Fourier transform infrared microspectroscopy as a tool to identify macroalgal propagules. / Bellgrove, Alecia; Kihara, Hiroshi; Iwata, Akira; Aoki, Masakazu N; Heraud, Philip Robert.

    In: Journal of Phycology, Vol. 45, No. 3, 2009, p. 560 - 570.

    Research output: Contribution to journalArticleResearchpeer-review

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    AU - Kihara, Hiroshi

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    AU - Heraud, Philip Robert

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