Formation and cell translocation of carbon nanotube-fibrinogen protein corona

Ran Chen; Slaven Radic; Poonam Choudhary; Kimberley G. Ledwell; George Guo Quan Huang; Jared M. Brown; Pu Chun Ke

doi:10.1063/1.4756794

Formation and cell translocation of carbon nanotube-fibrinogen protein corona

Ran Chen, Slaven Radic, Poonam Choudhary, Kimberley G. Ledwell, George Guo Quan Huang, Jared M. Brown, Pu Chun Ke

Research output: Contribution to journal › Article › Research › peer-review

13 Citations (Scopus)

Abstract

The binding of plasma fibrinogen with both single-walled and multi-walled carbon nanotubes (SWNTs and MWNTs) has been examined. Specifically, our absorbance study indicated that MWNTs were coated with multi-layers of fibrinogen to render a "hard protein corona," while SWNTs were adsorbed with thin layers of the protein to precipitate out of the aqueous phase. In addition, static quenching as a result of energy transfer from fluorescently labeled fibrinogen to their nanotube substrates was revealed by Stern-Volmer analysis. When exposed to HT-29 cells, the nanotubes and fibrinogen could readily dissociate, possibly stemming from their differential affinities for the amphiphilic membrane bilayer.

Original language	English
Article number	133702
Journal	Applied Physics Letters
Volume	101
Issue number	13
DOIs	https://doi.org/10.1063/1.4756794
Publication status	Published - 24 Sep 2012
Externally published	Yes

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10.1063/1.4756794

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Cite this

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title = "Formation and cell translocation of carbon nanotube-fibrinogen protein corona",

abstract = "The binding of plasma fibrinogen with both single-walled and multi-walled carbon nanotubes (SWNTs and MWNTs) has been examined. Specifically, our absorbance study indicated that MWNTs were coated with multi-layers of fibrinogen to render a {"}hard protein corona,{"} while SWNTs were adsorbed with thin layers of the protein to precipitate out of the aqueous phase. In addition, static quenching as a result of energy transfer from fluorescently labeled fibrinogen to their nanotube substrates was revealed by Stern-Volmer analysis. When exposed to HT-29 cells, the nanotubes and fibrinogen could readily dissociate, possibly stemming from their differential affinities for the amphiphilic membrane bilayer.",

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T1 - Formation and cell translocation of carbon nanotube-fibrinogen protein corona

AU - Chen, Ran

AU - Radic, Slaven

AU - Choudhary, Poonam

AU - Ledwell, Kimberley G.

AU - Huang, George Guo Quan

AU - Brown, Jared M.

AU - Ke, Pu Chun

PY - 2012/9/24

Y1 - 2012/9/24

N2 - The binding of plasma fibrinogen with both single-walled and multi-walled carbon nanotubes (SWNTs and MWNTs) has been examined. Specifically, our absorbance study indicated that MWNTs were coated with multi-layers of fibrinogen to render a "hard protein corona," while SWNTs were adsorbed with thin layers of the protein to precipitate out of the aqueous phase. In addition, static quenching as a result of energy transfer from fluorescently labeled fibrinogen to their nanotube substrates was revealed by Stern-Volmer analysis. When exposed to HT-29 cells, the nanotubes and fibrinogen could readily dissociate, possibly stemming from their differential affinities for the amphiphilic membrane bilayer.

AB - The binding of plasma fibrinogen with both single-walled and multi-walled carbon nanotubes (SWNTs and MWNTs) has been examined. Specifically, our absorbance study indicated that MWNTs were coated with multi-layers of fibrinogen to render a "hard protein corona," while SWNTs were adsorbed with thin layers of the protein to precipitate out of the aqueous phase. In addition, static quenching as a result of energy transfer from fluorescently labeled fibrinogen to their nanotube substrates was revealed by Stern-Volmer analysis. When exposed to HT-29 cells, the nanotubes and fibrinogen could readily dissociate, possibly stemming from their differential affinities for the amphiphilic membrane bilayer.

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