Formation and cell translocation of carbon nanotube-fibrinogen protein corona

Ran Chen, Slaven Radic, Poonam Choudhary, Kimberley G. Ledwell, George Guo Quan Huang, Jared M. Brown, Pu Chun Ke

Research output: Contribution to journalArticleResearchpeer-review

15 Citations (Scopus)


The binding of plasma fibrinogen with both single-walled and multi-walled carbon nanotubes (SWNTs and MWNTs) has been examined. Specifically, our absorbance study indicated that MWNTs were coated with multi-layers of fibrinogen to render a "hard protein corona," while SWNTs were adsorbed with thin layers of the protein to precipitate out of the aqueous phase. In addition, static quenching as a result of energy transfer from fluorescently labeled fibrinogen to their nanotube substrates was revealed by Stern-Volmer analysis. When exposed to HT-29 cells, the nanotubes and fibrinogen could readily dissociate, possibly stemming from their differential affinities for the amphiphilic membrane bilayer.

Original languageEnglish
Article number133702
JournalApplied Physics Letters
Issue number13
Publication statusPublished - 24 Sept 2012
Externally publishedYes

Cite this