Formation and cell translocation of carbon nanotube-fibrinogen protein corona

Ran Chen, Slaven Radic, Poonam Choudhary, Kimberley G. Ledwell, George Guo Quan Huang, Jared M. Brown, Pu Chun Ke

Research output: Contribution to journalArticleResearchpeer-review

13 Citations (Scopus)

Abstract

The binding of plasma fibrinogen with both single-walled and multi-walled carbon nanotubes (SWNTs and MWNTs) has been examined. Specifically, our absorbance study indicated that MWNTs were coated with multi-layers of fibrinogen to render a "hard protein corona," while SWNTs were adsorbed with thin layers of the protein to precipitate out of the aqueous phase. In addition, static quenching as a result of energy transfer from fluorescently labeled fibrinogen to their nanotube substrates was revealed by Stern-Volmer analysis. When exposed to HT-29 cells, the nanotubes and fibrinogen could readily dissociate, possibly stemming from their differential affinities for the amphiphilic membrane bilayer.

Original languageEnglish
Article number133702
JournalApplied Physics Letters
Volume101
Issue number13
DOIs
Publication statusPublished - 24 Sep 2012
Externally publishedYes

Cite this