Following aptamer-thrombin binding by force measurements

Bernhard Basnar, Roey Elnathan, Itamar Willner

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Abstract

The rupture forces between an aptamer (1)-functionalized AFM tip and a thrombin-modified Au surface are analyzed. The rupture force for a single aptamer/thrombin complex is determined as ̃4.45 pN. The analysis of the system reveals that the rupture forces correspond to the melting of the G-quadruplex structure of the aptamer bound to the thrombin. This melting of the G-quadruplex leads to the dissociation of the aptamer/thrombin complex.

Original languageEnglish
Pages (from-to)3638-3642
Number of pages5
JournalAnalytical Chemistry
Volume78
Issue number11
DOIs
Publication statusPublished - 1 Jun 2006
Externally publishedYes

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