Focal plane array infrared imaging: a new way to analyse leaf tissue

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Abstract

Here, a new approach to macromolecular imaging of leaf tissue using a multichannel focal plane array (FPA) infrared detector was compared with the proven method of infrared mapping with a synchrotron source, using transverse sections of leaves from a species of Eucalyptus. A new histological method was developed, ideally suited to infrared spectroscopic analysis of leaf tissue. Spatial resolution and the signal-to-noise ratio of the FPA imaging and synchrotron mapping methods were compared. An area of tissue 350 mu m(2) required approx. 8 h to map using the synchrotron technique and approx. 2 min to image using the FPA. The two methods produced similar infrared images, which differentiated all tissue types in the leaves according to their macromolecular chemistry. The synchrotron and FPA methods produced similar results, with the synchrotron method having superior signal-to-noise ratio and potentially better spatial resolution, whereas the FPA method had the advantage in terms of data acquisition time, expense and ease of use. FPA imaging offers a convenient, laboratory-based approach to microscopic chemical imaging of leaves.
Original languageEnglish
Pages (from-to)216 - 225
Number of pages10
JournalNew Phytologist
Volume173
Issue number1
Publication statusPublished - 2007

Cite this

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title = "Focal plane array infrared imaging: a new way to analyse leaf tissue",
abstract = "Here, a new approach to macromolecular imaging of leaf tissue using a multichannel focal plane array (FPA) infrared detector was compared with the proven method of infrared mapping with a synchrotron source, using transverse sections of leaves from a species of Eucalyptus. A new histological method was developed, ideally suited to infrared spectroscopic analysis of leaf tissue. Spatial resolution and the signal-to-noise ratio of the FPA imaging and synchrotron mapping methods were compared. An area of tissue 350 mu m(2) required approx. 8 h to map using the synchrotron technique and approx. 2 min to image using the FPA. The two methods produced similar infrared images, which differentiated all tissue types in the leaves according to their macromolecular chemistry. The synchrotron and FPA methods produced similar results, with the synchrotron method having superior signal-to-noise ratio and potentially better spatial resolution, whereas the FPA method had the advantage in terms of data acquisition time, expense and ease of use. FPA imaging offers a convenient, laboratory-based approach to microscopic chemical imaging of leaves.",
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Focal plane array infrared imaging: a new way to analyse leaf tissue. / Heraud, Philip Robert; Caine, Sally; Sanson, Gordon Drummond; Gleadow, Roslyn Margaret; Wood, Bayden Robert; McNaughton, Donald.

In: New Phytologist, Vol. 173, No. 1, 2007, p. 216 - 225.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Focal plane array infrared imaging: a new way to analyse leaf tissue

AU - Heraud, Philip Robert

AU - Caine, Sally

AU - Sanson, Gordon Drummond

AU - Gleadow, Roslyn Margaret

AU - Wood, Bayden Robert

AU - McNaughton, Donald

PY - 2007

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AB - Here, a new approach to macromolecular imaging of leaf tissue using a multichannel focal plane array (FPA) infrared detector was compared with the proven method of infrared mapping with a synchrotron source, using transverse sections of leaves from a species of Eucalyptus. A new histological method was developed, ideally suited to infrared spectroscopic analysis of leaf tissue. Spatial resolution and the signal-to-noise ratio of the FPA imaging and synchrotron mapping methods were compared. An area of tissue 350 mu m(2) required approx. 8 h to map using the synchrotron technique and approx. 2 min to image using the FPA. The two methods produced similar infrared images, which differentiated all tissue types in the leaves according to their macromolecular chemistry. The synchrotron and FPA methods produced similar results, with the synchrotron method having superior signal-to-noise ratio and potentially better spatial resolution, whereas the FPA method had the advantage in terms of data acquisition time, expense and ease of use. FPA imaging offers a convenient, laboratory-based approach to microscopic chemical imaging of leaves.

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JF - New Phytologist

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