Flux balance analysis as an alternative method to estimate fluxes without labeling

Eva Grafahrend-Belau, Astrid Junker, Falk Schreiber, Bjorn Junker

    Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Other

    2 Citations (Scopus)

    Abstract

    The analysis of plant metabolic networks essentially contributes to the understanding of the efficiency of plant systems in terms of their biotechnological usage. Metabolic fluxes are determined by biochemical parameters such as metabolite concentrations as well as enzyme properties and activities, which in turn are the result of various regulatory events at various levels between control of transcription and posttranslational regulation of enzyme protein activity. Thus, knowledge about metabolic fluxes on a large scale provides an integrated view on the functional state of a metabolically active cell, organ, or system. In this chapter, we introduce flux balance analysis as a constraint-based method for the prediction of optimal metabolic fluxes in a given metabolic network. Furthermore, we provide a step-by-step protocol for metabolic network reconstruction and constraint-based analysis using the COBRA Toolbox. © Springer Science+Business Media New York 2014.

    Original languageEnglish
    Title of host publicationPlant Metabolic Flux Analysis: Methods and Protocols
    EditorsMartine Dieuaide-Noubhani, Ana Paula Alonso
    Place of PublicationNew York NY USA
    PublisherHumana Press
    Pages281-299
    Number of pages19
    Volume1090
    ISBN (Print)9781627036870
    DOIs
    Publication statusPublished - 2014

    Publication series

    NameMethods in Molecular Biology
    Volume1090
    ISSN (Print)10643745

    Keywords

    • COBRA toolbox
    • Flux balance analysis
    • Matlab
    • Metabolic network reconstruction

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