Fluorophore

Dendrimer ratio impacts cellular uptake and intracellular fluorescence lifetime

Casey A. Dougherty, Sriram Vaidyanathan, Bradford G. Orr, Mark M. Banaszak Holl

Research output: Contribution to journalArticleResearchpeer-review

Abstract

G5-NH2-TAMRAn (n = 1-4, 5+, and 1.5avg) were prepared with n = 1-4 as a precise dye:dendrimer ratio, 5+ as a mixture of dendrimers with 5 or more dye per dendrimer, and 1.5avg as a Poisson distribution of dye:dendrimer ratios with a mean of 1.5 dye per dendrimer. The absorption intensity increased sublinearly with n whereas the fluorescence emission and lifetime decreased with an increasing number of dyes per dendrimer. Flow cytometry was employed to quantify uptake into HEK293A cells. Dendrimers with 2-4 dyes were found to have greater uptake than dendrimer with a single dye. Fluorescence lifetime imaging microscopy (FLIM) showed that the different dye:dendrimer ratio alone was sufficient to change the fluorescence lifetime of the material observed inside cells. We also observed that the lifetime of G5-NH2-TAMRA5+ increased when present in the cell as compared to solution. However, cells treated with G5-NH2-TAMRA1.5avg did not exhibit the high lifetime components present in G5-NH2-TAMRA1 and G5-NH2-TAMRA5+. In general, the effects of the dye:dendrimer ratio on fluorescence lifetime were of similar magnitude to environmentally induced lifetime shifts.

Original languageEnglish
Pages (from-to)304-315
Number of pages12
JournalBioconjugate Chemistry
Volume26
Issue number2
DOIs
Publication statusPublished - 18 Feb 2015
Externally publishedYes

Cite this

Dougherty, Casey A. ; Vaidyanathan, Sriram ; Orr, Bradford G. ; Banaszak Holl, Mark M. / Fluorophore : Dendrimer ratio impacts cellular uptake and intracellular fluorescence lifetime. In: Bioconjugate Chemistry. 2015 ; Vol. 26, No. 2. pp. 304-315.
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Fluorophore : Dendrimer ratio impacts cellular uptake and intracellular fluorescence lifetime. / Dougherty, Casey A.; Vaidyanathan, Sriram; Orr, Bradford G.; Banaszak Holl, Mark M.

In: Bioconjugate Chemistry, Vol. 26, No. 2, 18.02.2015, p. 304-315.

Research output: Contribution to journalArticleResearchpeer-review

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T2 - Dendrimer ratio impacts cellular uptake and intracellular fluorescence lifetime

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