Fluoride enhances transfection activity of carbonate apatite by increasing cytoplasmic stability of plasmid DNA

Md Ezharul Chowdhury

    Research output: Contribution to journalArticleResearchpeer-review

    6 Citations (Scopus)

    Abstract

    Recently, we have developed pH-sensing inorganic nanocrystals of carbonate apatite for efficient and cell-targeted delivery of gene and gene-silencing RNA. Here we show that addition of very low level of fluoride to the particleforming medium facilitates a robust increase in transgene expression following post-incubation of the particles with HeLa cells. Confocal microscopic observation and Southern blotting prove the cytoplasmic existence of plasmid DNA delivered by likely formed fluoridated carbonate apatite particles while degradation of plasmid DNA presumably by cytoplasmic nucleases was noticed following delivery with apatite particles alone.
    Original languageEnglish
    Pages (from-to)745 - 747
    Number of pages3
    JournalBiochemical and Biophysical Research Communications
    Volume409
    Issue number4
    DOIs
    Publication statusPublished - 2011

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