Fluorescent quantitation method for differentiating the nativity of green fluorescent protein

Few Ne Chew; Wen Siang Tan; Beng Ti Tey

doi:10.1016/j.jbiosc.2010.10.004

Fluorescent quantitation method for differentiating the nativity of green fluorescent protein

Few Ne Chew, Wen Siang Tan, Beng Ti Tey

Research output: Contribution to journal › Article › Research › peer-review

1 Citation (Scopus)

Abstract

A gel imaging method was employed to quantitate the GFP that had been subjected to denaturation and degradation treatments. This method is able to differentiate the nativity of GFP by relating the observed changes in the position of fluorescent bands which is unable to be detected using the spectrofluorometric method.

Original language	English
Pages (from-to)	246-248
Number of pages	3
Journal	Journal of Bioscience and Bioengineering
Volume	111
Issue number	2
DOIs	https://doi.org/10.1016/j.jbiosc.2010.10.004
Publication status	Published - Feb 2011
Externally published	Yes

Keywords

Degradation
Denaturation
Fluorescence quantitation
Gel imaging method
Green fluorescent protein
Nativity
Spectrofluorometer

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10.1016/j.jbiosc.2010.10.004

Cite this

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title = "Fluorescent quantitation method for differentiating the nativity of green fluorescent protein",

abstract = "A gel imaging method was employed to quantitate the GFP that had been subjected to denaturation and degradation treatments. This method is able to differentiate the nativity of GFP by relating the observed changes in the position of fluorescent bands which is unable to be detected using the spectrofluorometric method.",

keywords = "Degradation, Denaturation, Fluorescence quantitation, Gel imaging method, Green fluorescent protein, Nativity, Spectrofluorometer",

author = "Chew, \{Few Ne\} and Tan, \{Wen Siang\} and Tey, \{Beng Ti\}",

note = "Funding Information: This study was supported by the RUGS Grant ( 05-01-09-0786RU ) from Universiti Putra Malaysia . Few Ne Chew is supported by the Graduate Research Fellowship from Universiti Putra Malaysia. Copyright: Copyright 2011 Elsevier B.V., All rights reserved.",

year = "2011",

month = feb,

doi = "10.1016/j.jbiosc.2010.10.004",

language = "English",

volume = "111",

pages = "246--248",

journal = "Journal of Bioscience and Bioengineering",

issn = "1389-1723",

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T1 - Fluorescent quantitation method for differentiating the nativity of green fluorescent protein

AU - Chew, Few Ne

AU - Tan, Wen Siang

AU - Tey, Beng Ti

N1 - Funding Information: This study was supported by the RUGS Grant ( 05-01-09-0786RU ) from Universiti Putra Malaysia . Few Ne Chew is supported by the Graduate Research Fellowship from Universiti Putra Malaysia. Copyright: Copyright 2011 Elsevier B.V., All rights reserved.

PY - 2011/2

Y1 - 2011/2

N2 - A gel imaging method was employed to quantitate the GFP that had been subjected to denaturation and degradation treatments. This method is able to differentiate the nativity of GFP by relating the observed changes in the position of fluorescent bands which is unable to be detected using the spectrofluorometric method.

AB - A gel imaging method was employed to quantitate the GFP that had been subjected to denaturation and degradation treatments. This method is able to differentiate the nativity of GFP by relating the observed changes in the position of fluorescent bands which is unable to be detected using the spectrofluorometric method.

KW - Degradation

KW - Denaturation

KW - Fluorescence quantitation

KW - Gel imaging method

KW - Green fluorescent protein

KW - Nativity

KW - Spectrofluorometer

UR - https://www.scopus.com/pages/publications/78951490896

U2 - 10.1016/j.jbiosc.2010.10.004

DO - 10.1016/j.jbiosc.2010.10.004

M3 - Article

C2 - 21036662

AN - SCOPUS:78951490896

SN - 1389-1723

VL - 111

SP - 246

EP - 248

JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

IS - 2

ER -

Fluorescent quantitation method for differentiating the nativity of green fluorescent protein

Abstract

Keywords

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