Fluorescent quantitation method for differentiating the nativity of green fluorescent protein

Few Ne Chew, Wen Siang Tan, Beng Ti Tey

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Abstract

A gel imaging method was employed to quantitate the GFP that had been subjected to denaturation and degradation treatments. This method is able to differentiate the nativity of GFP by relating the observed changes in the position of fluorescent bands which is unable to be detected using the spectrofluorometric method.

Original languageEnglish
Pages (from-to)246-248
Number of pages3
JournalJournal of Bioscience and Bioengineering
Volume111
Issue number2
DOIs
Publication statusPublished - Feb 2011
Externally publishedYes

Keywords

  • Degradation
  • Denaturation
  • Fluorescence quantitation
  • Gel imaging method
  • Green fluorescent protein
  • Nativity
  • Spectrofluorometer

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