Fluorescent Labeling and Biodistribution of Latex Nanoparticles Formed by Surfactant-Free RAFT Emulsion Polymerization

Cheuk Ka Poon, Owen Tang, Xin Ming Chen, Byung Kim, Matthias Hartlieb, Carol A. Pollock, Brian S. Hawkett, Sebastien Perrier

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12 Citations (Scopus)

Abstract

The authors report the preparation of a novel range of functional polyacrylamide stabilized polystyrene nanoparticles, obtained by surfactant-free reversible addition-fragmentation chain transfer (RAFT) emulsion polymerization, their fluorescent tagging, cellular uptake, and biodistribution. The authors show the versatility of the RAFT emulsion process for the design of functional nanoparticles of well-defined size that can be used as drug delivery vectors. Functionalization with a fluorescent tag offers a useful visualization tool for tracing, localization, and clearance studies of these carriers in biological models. The studies are carried out by labeling the sterically stabilized latex particles chemically with rhodamine B. The fluorescent particles are incubated in a healthy human renal proximal tubular cell line model, and intravenously injected into a mouse model. Cellular localization and biodistribution of these particles on the biological models are explored.

Original languageEnglish
Article number1600366
Number of pages10
JournalMacromolecular Bioscience
Volume17
Issue number10
DOIs
Publication statusPublished - Oct 2017
Externally publishedYes

Keywords

  • Biodistribution
  • Cell uptake
  • Nanoparticles
  • RAFT emulsion

Cite this

Poon, C. K., Tang, O., Chen, X. M., Kim, B., Hartlieb, M., Pollock, C. A., Hawkett, B. S., & Perrier, S. (2017). Fluorescent Labeling and Biodistribution of Latex Nanoparticles Formed by Surfactant-Free RAFT Emulsion Polymerization. Macromolecular Bioscience, 17(10), [1600366]. https://doi.org/10.1002/mabi.201600366