Fluorescence photobleaching analysis for the study of cellular dynamics

Nectarios Klonis, Melanie Rug, Ian Steward Harper, Mark Wickham, Alan F Cowman, Leann Tilley

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Abstract

The wide availability of the confocal microscope and the emergence of green fluorescent protein (GFP) transfection technology has led to the increasing use of photobleaching studies to examine aspects of cellular dynamics in living cells. In this review, we examine the theory and practice of performing photobleaching studies using a confocal microscope. We illustrate the application of photobleaching protocols using our own measurements of fluorescently labelled red blood cells and of malaria parasite-infected erythrocytes expressing GFP fusions and examine other examples from the literature.
Original languageEnglish
Pages (from-to)36 - 51
Number of pages16
JournalEuropean Biophysics Journal
Volume31
Issue number1
Publication statusPublished - 2002

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