Fluorescence Measurement Techniques

Sven Beer; Mats Björk; John Beardall

doi:10.1007/978-981-15-5354-7_26

Fluorescence Measurement Techniques

Sven Beer, Mats Björk, John Beardall

School of Biological Sciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

Abstract

Chlorophyll fluorometers are constructed such that the immediate outcomes of the measurements provide information on quantum yields (Y) of electrons passing through photosystem II (PSII). If the photosynthetically active radiation (PAR) absorbed by PSII (PARa) is known, then photosynthetic rates can be calculated as electron transport rates (ETR). If only the incident irradiance (PARi) is known (which often is the case), then relative ETRs (rETR) can be calculated as the product of Y and PARi. There are sometimes easy ways to at least estimate PARa, and they will be described in this chapter, as will other practical means to measure photosynthetic parameters using fluorometry.

Original language	English
Title of host publication	Research Methods of Environmental Physiology in Aquatic Sciences
Editors	Kunshan Gao, David A Hutchins, John Beardall
Place of Publication	Singapore
Publisher	Springer
Chapter	26
Pages	231-238
Number of pages	8
ISBN (Electronic)	9789811553547
ISBN (Print)	9789811553530
DOIs	https://doi.org/10.1007/978-981-15-5354-7_26
Publication status	Published - 2021

Keywords

Aquatic plants
PAM fluorometers
PAM measurements
Photosynthesis
Quantum yield

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10.1007/978-981-15-5354-7_26

Cite this

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N2 - Chlorophyll fluorometers are constructed such that the immediate outcomes of the measurements provide information on quantum yields (Y) of electrons passing through photosystem II (PSII). If the photosynthetically active radiation (PAR) absorbed by PSII (PARa) is known, then photosynthetic rates can be calculated as electron transport rates (ETR). If only the incident irradiance (PARi) is known (which often is the case), then relative ETRs (rETR) can be calculated as the product of Y and PARi. There are sometimes easy ways to at least estimate PARa, and they will be described in this chapter, as will other practical means to measure photosynthetic parameters using fluorometry.

AB - Chlorophyll fluorometers are constructed such that the immediate outcomes of the measurements provide information on quantum yields (Y) of electrons passing through photosystem II (PSII). If the photosynthetically active radiation (PAR) absorbed by PSII (PARa) is known, then photosynthetic rates can be calculated as electron transport rates (ETR). If only the incident irradiance (PARi) is known (which often is the case), then relative ETRs (rETR) can be calculated as the product of Y and PARi. There are sometimes easy ways to at least estimate PARa, and they will be described in this chapter, as will other practical means to measure photosynthetic parameters using fluorometry.

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KW - PAM fluorometers

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Fluorescence Measurement Techniques

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