Fluctuating expression of microRNAs in adenovirus infected cells

Hongxing Zhao, Maoshan Chen, Christian Tellgren-Roth, Ulf Pettersson

Research output: Contribution to journalArticleResearchpeer-review

30 Citations (Scopus)

Abstract

The changes in cellular microRNA (miRNA) expression during the course of an adenovirus type 2 infection in human lung fibroblast were studied by deep RNA sequencing. Expressions of 175 miRNAs with over 100 transcripts per million nucleotides were changed more than 1.5-fold. The expression patterns of these miRNAs changed dramatically during the course of the infection, from upregulation of the miRNAs known as tumor suppressors (such as miR-22, miR-320, let-7, miR-181b, and miR-155) and down-regulation of oncogenic miRNAs (such as miR-21 and miR-31) early to downregulation of tumor suppressor miRNAs (such as let-7 family, mir-30 family, 23/27 cluster) and upregulation of oncogenic miRNAs (include miR-125, miR-27, miR-191) late after infection. The switch in miRNA expression pattern occurred when adenovirus DNA replication started. Furthermore, deregulation of cellular miRNA expression was a step-wise and special sets of miRNAs were deregulated in different phases of infection.

Original languageEnglish
Pages (from-to)99-111
Number of pages13
JournalVirology
Volume478
DOIs
Publication statusPublished - 1 Apr 2015
Externally publishedYes

Keywords

  • Adenovirus infection
  • Cellular microRNA
  • Deep sequencing
  • Expression profile

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