Flow-induced protein kinase A-CREB pathway acts via BMP signaling to promote HSC emergence

Peter Geon Kim, Haruko Nakano, Partha P. Das, Michael J. Chen, R. Grant Rowe, Stephanie S. Chou, Samantha J. Ross, Kathleen M. Sakamoto, Leonard I. Zon, Thorsten M. Schlaeger, Stuart H. Orkin, Atsushi Nakano, George Q. Daley

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35 Citations (Scopus)

Abstract

Fluid shear stress promotes the emergence of hematopoietic stem cells (HSCs) in the aorta- gonad-mesonephros (AGM) of the developing mouse embryo. We determined that the AGM is enriched for expression of targets of protein kinase A (PKA)-cAMP response elementbinding protein (CREB), a pathway activated by fluid shear stress. By analyzing CREB genomic occupancy from chromatin-immunoprecipitation sequencing (ChIP-seq) data, we identified the bone morphogenetic protein (BMP) pathway as a potential regulator of CREB. By chemical modulation of the PKA-CREB and BMP pathways in isolated AGM VEcadherin+ cells from mid-gestation embryos, we demonstrate that PKA-CREB regulates hematopoietic engraftment and clonogenicity of hematopoietic progenitors, and is dependent on secreted BMP ligands through the type I BMP receptor. Finally, we observed blunting of this signaling axis using Ncx1-null embryos, which lack a heartbeat and intravascular flow. Collectively, we have identified a novel PKA-CREB-BMP signaling pathway downstream of shear stress that regulates HSC emergence in the AGM via the endothelial-tohematopoietic transition.

Original languageEnglish
Pages (from-to)633-648
Number of pages16
JournalJournal of Experimental Medicine
Volume212
Issue number5
DOIs
Publication statusPublished - 4 May 2015
Externally publishedYes

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