Flow Cytometry Phenotyping of Bone Marrow-Derived Macrophages from Wild-Type and Mif-/- Mice

Jacqueline K. Flynn; Nadia S. Deen; James Harris

doi:10.1007/978-1-4939-9936-1_6

Flow Cytometry Phenotyping of Bone Marrow-Derived Macrophages from Wild-Type and Mif^-/- Mice

Jacqueline K. Flynn, Nadia S. Deen, James Harris

Centre for Inflammatory Disease Monash Health

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

2 Citations (Scopus)

Abstract

Phenotyping cells by flow cytometry is a powerful way to identify cell type and any morphological changes during cell culture. The staining procedure used in this chapter enables the characterization of mouse macrophages by a flow cytometry antibody panel which can be used for both bone marrow-derived macrophages (BMM) and macrophages derived from other tissues, such as the mouse spleen or peritoneal cavity. The surface and intracellular staining methods are versatile and can be applied to flow cytometry staining of several different cell types by changing the surface markers used with knowledge of which receptors are expressed on different cell types.

Original language	English
Title of host publication	Macrophage Migration Inhibitory Factor
Subtitle of host publication	Methods and Protocols
Editors	James Harris, Eric Morand
Place of Publication	New York, NY
Publisher	Humana Press
Chapter	6
Pages	57-66
Number of pages	10
ISBN (Electronic)	9781493999361
ISBN (Print)	9781493999354
DOIs	https://doi.org/10.1007/978-1-4939-9936-1_6
Publication status	Published - 1 Jan 2020

Publication series

Name	Methods in Molecular Biology: Springer Protocols
Publisher	Springer
Volume	2080
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

BMM
Cell surface receptors
Flow cytometry
Fluorochromes
Macrophages
MIF

Access to Document

10.1007/978-1-4939-9936-1_6

Cite this

Flynn, J. K., Deen, N. S., & Harris, J. (2020). Flow Cytometry Phenotyping of Bone Marrow-Derived Macrophages from Wild-Type and Mif^-/- Mice. In J. Harris, & E. Morand (Eds.), Macrophage Migration Inhibitory Factor: Methods and Protocols (pp. 57-66). (Methods in Molecular Biology: Springer Protocols; Vol. 2080). Humana Press. https://doi.org/10.1007/978-1-4939-9936-1_6

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Flow Cytometry Phenotyping of Bone Marrow-Derived Macrophages from Wild-Type and Mif^-/- Mice. / Flynn, Jacqueline K.; Deen, Nadia S.; Harris, James.
Macrophage Migration Inhibitory Factor: Methods and Protocols. ed. / James Harris; Eric Morand. New York, NY: Humana Press, 2020. p. 57-66 (Methods in Molecular Biology: Springer Protocols; Vol. 2080).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (Book) › Other › peer-review

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