Flow Cytometry Phenotyping of Bone Marrow-Derived Macrophages from Wild-Type and Mif-/- Mice

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Phenotyping cells by flow cytometry is a powerful way to identify cell type and any morphological changes during cell culture. The staining procedure used in this chapter enables the characterization of mouse macrophages by a flow cytometry antibody panel which can be used for both bone marrow-derived macrophages (BMM) and macrophages derived from other tissues, such as the mouse spleen or peritoneal cavity. The surface and intracellular staining methods are versatile and can be applied to flow cytometry staining of several different cell types by changing the surface markers used with knowledge of which receptors are expressed on different cell types.

Original languageEnglish
Title of host publicationMacrophage Migration Inhibitory Factor
Subtitle of host publicationMethods and Protocols
EditorsJames Harris, Eric Morand
Place of PublicationNew York, NY
PublisherHumana Press
Number of pages10
ISBN (Electronic)9781493999361
ISBN (Print)9781493999354
Publication statusPublished - 1 Jan 2020

Publication series

NameMethods in Molecular Biology: Springer Protocols
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • BMM
  • Cell surface receptors
  • Flow cytometry
  • Fluorochromes
  • Macrophages
  • MIF

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