Flow Cytometry Phenotyping of Bone Marrow-Derived Macrophages from Wild-Type and Mif-/- Mice

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

Abstract

Phenotyping cells by flow cytometry is a powerful way to identify cell type and any morphological changes during cell culture. The staining procedure used in this chapter enables the characterization of mouse macrophages by a flow cytometry antibody panel which can be used for both bone marrow-derived macrophages (BMM) and macrophages derived from other tissues, such as the mouse spleen or peritoneal cavity. The surface and intracellular staining methods are versatile and can be applied to flow cytometry staining of several different cell types by changing the surface markers used with knowledge of which receptors are expressed on different cell types.

Original languageEnglish
Title of host publicationMacrophage Migration Inhibitory Factor
Subtitle of host publicationMethods and Protocols
EditorsJames Harris, Eric Morand
Place of PublicationNew York, NY
PublisherHumana Press
Chapter6
Pages57-66
Number of pages10
ISBN (Electronic)9781493999361
ISBN (Print)9781493999354
DOIs
Publication statusPublished - 1 Jan 2020

Publication series

NameMethods in Molecular Biology: Springer Protocols
PublisherSpringer
Volume2080
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • BMM
  • Cell surface receptors
  • Flow cytometry
  • Fluorochromes
  • Macrophages
  • MIF

Cite this

Flynn, J. K., Deen, N. S., & Harris, J. (2020). Flow Cytometry Phenotyping of Bone Marrow-Derived Macrophages from Wild-Type and Mif-/- Mice. In J. Harris, & E. Morand (Eds.), Macrophage Migration Inhibitory Factor: Methods and Protocols (pp. 57-66). (Methods in Molecular Biology: Springer Protocols; Vol. 2080). New York, NY: Humana Press. https://doi.org/10.1007/978-1-4939-9936-1_6