Flow Cytometric Analysis of Human Embryonic Stem Cells

Andrew L. Laslett, Andrew Fryga, Martin F. Pera

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Researchpeer-review

3 Citations (Scopus)

Abstract

Flow cytometric techniques are used to quantify, and separate subpopulations of cells contained within complex cell mixtures. This is accomplished by exploiting the differences in the basic optical properties of the cells, in their specific immuno-reactivity to different antibodies or combinations of antibodies, or in their physiological or biochemical properties. In a flow cytometer, cells in suspension are made to flow one at a time by a light source, typically a laser, with the resulting light scatter yielding information about their size and cellular complexity. Cells can be labeled with flurophore-tagged antibodies or loaded with multiple dyes, with the emitted fluorescence estimating cellular parameters such as nucleic acid content, membrane potential, and density of membrane-bound antigens.

Original languageEnglish
Title of host publicationHuman Stem Cell Manual
PublisherButterworth-Heinemann
Pages96-107
Number of pages12
ISBN (Print)9780123704658
DOIs
Publication statusPublished - 2007

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