Fission yeast SWI/SNF and RSC complexes show compositional and functional differences from budding yeast

Brendon J. Monahan, Judit Villén, Samuel Marguerat, Jürg Bähler, Steven P. Gygi, Fred Winston

Research output: Contribution to journalArticleResearchpeer-review

48 Citations (Scopus)

Abstract

SWI/SNF chromatin-remodeling complexes have crucial roles in transcription and other chromatin-related processes. The analysis of the two members of this class in Saccharomyces cerevisiae, SWI/SNF and RSC, has heavily contributed to our understanding of these complexes. To understand the in vivo functions of SWI/SNF and RSC in an evolutionarily distant organism, we have characterized these complexes in Schizosaccharomyces pombe. Although core components are conserved between the two yeasts, the compositions of S. pombe SWI/SNF and RSC differ from their S. cerevisiae counterparts and in some ways are more similar to metazoan complexes. Furthermore, several of the conserved proteins, including actin-like proteins, are markedly different between the two yeasts with respect to their requirement for viability. Finally, phenotypic and microarray analyses identified widespread requirements for SWI/SNF and RSC on transcription including strong evidence that SWI/SNF directly represses iron-transport genes.

Original languageEnglish
Pages (from-to)873-880
Number of pages8
JournalNature Structural & Molecular Biology
Volume15
Issue number8
DOIs
Publication statusPublished - Aug 2008
Externally publishedYes

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