Fine structure of neurally differentiated iPS cells generated from a multiple sclerosis (MS) patient: a case study

Daniella Herszfeld, Natalie Lisa Payne, Aude Sylvain, Guizhi Sun, Claude Charles Andre Bernard, Joan Marea Clark, Arunachalam Henry Sathananthan

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We compared the characteristics of neural cells derived from induced pluripotent stem (iPS) cells from a patient with multiple sclerosis versus neurally differentiated control iPS cells of a healthy individual. The iPS cells were differentiated toward the oligodendrocyte lineage using a four-step protocol established for the differentiation of embryonic stem cells. The resulting cell population was immunostained on day 112 of differentiation for the presence of oligodendrocytes and analyzed by transmission electron microscopy (TEM). Both patient and control samples resembled a mixed population of neural cells rather than oligodendroglia of high purity, including neural stem cell-like cells and possibly oligodendrocytes demonstrable by TEM.
Original languageEnglish
Pages (from-to)1869 - 1875
Number of pages7
JournalMicroscopy and Microanalysis
Issue number6
Publication statusPublished - 2014

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