Fine structure of neurally differentiated iPS cells generated from a multiple sclerosis (MS) patient: a case study

Daniella Herszfeld; Natalie Lisa Payne; Aude Sylvain; Guizhi Sun; Claude Charles Andre Bernard; Joan Marea Clark; Arunachalam Henry Sathananthan

doi:10.1017/S1431927614013312

Fine structure of neurally differentiated iPS cells generated from a multiple sclerosis (MS) patient: a case study

Daniella Herszfeld, Natalie Lisa Payne, Aude Sylvain, Guizhi Sun, Claude Charles Andre Bernard, Joan Marea Clark, Arunachalam Henry Sathananthan

Research output: Contribution to journal › Article › Research › peer-review

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Abstract

We compared the characteristics of neural cells derived from induced pluripotent stem (iPS) cells from a patient with multiple sclerosis versus neurally differentiated control iPS cells of a healthy individual. The iPS cells were differentiated toward the oligodendrocyte lineage using a four-step protocol established for the differentiation of embryonic stem cells. The resulting cell population was immunostained on day 112 of differentiation for the presence of oligodendrocytes and analyzed by transmission electron microscopy (TEM). Both patient and control samples resembled a mixed population of neural cells rather than oligodendroglia of high purity, including neural stem cell-like cells and possibly oligodendrocytes demonstrable by TEM.

Original language	English
Pages (from-to)	1869 - 1875
Number of pages	7
Journal	Microscopy and Microanalysis
Volume	20
Issue number	6
DOIs	https://doi.org/10.1017/S1431927614013312
Publication status	Published - 2014

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title = "Fine structure of neurally differentiated iPS cells generated from a multiple sclerosis (MS) patient: a case study",

abstract = "We compared the characteristics of neural cells derived from induced pluripotent stem (iPS) cells from a patient with multiple sclerosis versus neurally differentiated control iPS cells of a healthy individual. The iPS cells were differentiated toward the oligodendrocyte lineage using a four-step protocol established for the differentiation of embryonic stem cells. The resulting cell population was immunostained on day 112 of differentiation for the presence of oligodendrocytes and analyzed by transmission electron microscopy (TEM). Both patient and control samples resembled a mixed population of neural cells rather than oligodendroglia of high purity, including neural stem cell-like cells and possibly oligodendrocytes demonstrable by TEM.",

author = "Daniella Herszfeld and Payne, {Natalie Lisa} and Aude Sylvain and Guizhi Sun and Bernard, {Claude Charles Andre} and Clark, {Joan Marea} and Sathananthan, {Arunachalam Henry}",

year = "2014",

doi = "10.1017/S1431927614013312",

language = "English",

volume = "20",

pages = "1869 -- 1875",

journal = "Microscopy and Microanalysis",

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publisher = "Cambridge University Press",

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}

Fine structure of neurally differentiated iPS cells generated from a multiple sclerosis (MS) patient: a case study. / Herszfeld, Daniella; Payne, Natalie Lisa; Sylvain, Aude; Sun, Guizhi; Bernard, Claude Charles Andre; Clark, Joan Marea; Sathananthan, Arunachalam Henry.

In: Microscopy and Microanalysis, Vol. 20, No. 6, 2014, p. 1869 - 1875.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Fine structure of neurally differentiated iPS cells generated from a multiple sclerosis (MS) patient: a case study

AU - Herszfeld, Daniella

AU - Payne, Natalie Lisa

AU - Sylvain, Aude

AU - Sun, Guizhi

AU - Bernard, Claude Charles Andre

AU - Clark, Joan Marea

AU - Sathananthan, Arunachalam Henry

PY - 2014

Y1 - 2014

N2 - We compared the characteristics of neural cells derived from induced pluripotent stem (iPS) cells from a patient with multiple sclerosis versus neurally differentiated control iPS cells of a healthy individual. The iPS cells were differentiated toward the oligodendrocyte lineage using a four-step protocol established for the differentiation of embryonic stem cells. The resulting cell population was immunostained on day 112 of differentiation for the presence of oligodendrocytes and analyzed by transmission electron microscopy (TEM). Both patient and control samples resembled a mixed population of neural cells rather than oligodendroglia of high purity, including neural stem cell-like cells and possibly oligodendrocytes demonstrable by TEM.

AB - We compared the characteristics of neural cells derived from induced pluripotent stem (iPS) cells from a patient with multiple sclerosis versus neurally differentiated control iPS cells of a healthy individual. The iPS cells were differentiated toward the oligodendrocyte lineage using a four-step protocol established for the differentiation of embryonic stem cells. The resulting cell population was immunostained on day 112 of differentiation for the presence of oligodendrocytes and analyzed by transmission electron microscopy (TEM). Both patient and control samples resembled a mixed population of neural cells rather than oligodendroglia of high purity, including neural stem cell-like cells and possibly oligodendrocytes demonstrable by TEM.

UR - http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=9474292&fileId=S1431927614013312

U2 - 10.1017/S1431927614013312

DO - 10.1017/S1431927614013312

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EP - 1875

JO - Microscopy and Microanalysis

JF - Microscopy and Microanalysis

SN - 1431-9276

IS - 6

ER -

Fine structure of neurally differentiated iPS cells generated from a multiple sclerosis (MS) patient: a case study

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