TY - JOUR
T1 - Fine structure of neurally differentiated iPS cells generated from a multiple sclerosis (MS) patient: a case study
AU - Herszfeld, Daniella
AU - Payne, Natalie Lisa
AU - Sylvain, Aude
AU - Sun, Guizhi
AU - Bernard, Claude Charles Andre
AU - Clark, Joan Marea
AU - Sathananthan, Arunachalam Henry
PY - 2014
Y1 - 2014
N2 - We compared the characteristics of neural cells derived from induced pluripotent stem (iPS) cells from a patient with multiple sclerosis versus neurally differentiated control iPS cells of a healthy individual. The iPS cells were differentiated toward the oligodendrocyte lineage using a four-step protocol established for the differentiation of embryonic stem cells. The resulting cell population was immunostained on day 112 of differentiation for the presence of oligodendrocytes and analyzed by transmission electron microscopy (TEM). Both patient and control samples resembled a mixed population of neural cells rather than oligodendroglia of high purity, including neural stem cell-like cells and possibly oligodendrocytes demonstrable by TEM.
AB - We compared the characteristics of neural cells derived from induced pluripotent stem (iPS) cells from a patient with multiple sclerosis versus neurally differentiated control iPS cells of a healthy individual. The iPS cells were differentiated toward the oligodendrocyte lineage using a four-step protocol established for the differentiation of embryonic stem cells. The resulting cell population was immunostained on day 112 of differentiation for the presence of oligodendrocytes and analyzed by transmission electron microscopy (TEM). Both patient and control samples resembled a mixed population of neural cells rather than oligodendroglia of high purity, including neural stem cell-like cells and possibly oligodendrocytes demonstrable by TEM.
UR - http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=9474292&fileId=S1431927614013312
U2 - 10.1017/S1431927614013312
DO - 10.1017/S1431927614013312
M3 - Article
VL - 20
SP - 1869
EP - 1875
JO - Microscopy and Microanalysis
JF - Microscopy and Microanalysis
SN - 1431-9276
IS - 6
ER -