Field- and clinically derived estimates of Wolbachia-mediated blocking of dengue virus transmission potential in Aedes aegypti mosquitoes

Lauren B. Carrington, Bich Chau Nguyen Tran, Nhat Thanh Hoang Le, Tai Thi Hue Luong, Truong Thanh Nguyen, Phong Thanh Nguyen, Nguyen Van Vinh Chau , Huong Thi Cam Nguyen, Trung Tuan Vu, Long Thi Vo, Dui Thi Le, Nhu Tuyet Vu, Giang Thi Nguyen, Hung Quoc Luu, Anh Duc Dang, Timothy P. Hurst, Scott L. O’Neill, Vi Thuy Tran, Duong Thi Hue Kien, Nguyet Minh Nguyen & 3 others Marcel Wolbers, Bridget Wills, Cameron P. Simmons

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The wMel strain of Wolbachia can reduce the permissiveness of Aedes aegypti mosquitoes to disseminated arboviral infections. Here, we report that wMel-infected Ae. aegypti (Ho Chi Minh City background), when directly blood-fed on 141 viremic dengue patients, have lower dengue virus (DENV) transmission potential and have a longer extrinsic incubation period than their wild-type counterparts. The wMel-infected mosquitoes that are field-reared have even greater relative resistance to DENV infection when fed on patient-derived viremic blood meals. This is explained by an increased susceptibility of field-reared wild-type mosquitoes to infection than laboratory-reared counterparts. Collectively, these field- and clinically relevant findings support the continued careful field-testing of wMel introgression for the biocontrol of Ae. aegypti-born arboviruses.

Original languageEnglish
Pages (from-to)361-366
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume115
Issue number2
DOIs
Publication statusPublished - 9 Jan 2017

Keywords

  • Aedes aegypti mosquito
  • Dengue virus
  • Extrinsic incubation period
  • Virus transmission
  • WMel Wolbachia

Cite this

Carrington, Lauren B. ; Tran, Bich Chau Nguyen ; Le, Nhat Thanh Hoang ; Luong, Tai Thi Hue ; Nguyen, Truong Thanh ; Nguyen, Phong Thanh ; Chau , Nguyen Van Vinh ; Nguyen, Huong Thi Cam ; Vu, Trung Tuan ; Vo, Long Thi ; Le, Dui Thi ; Vu, Nhu Tuyet ; Nguyen, Giang Thi ; Luu, Hung Quoc ; Dang, Anh Duc ; Hurst, Timothy P. ; O’Neill, Scott L. ; Tran, Vi Thuy ; Kien, Duong Thi Hue ; Nguyen, Nguyet Minh ; Wolbers, Marcel ; Wills, Bridget ; Simmons, Cameron P. / Field- and clinically derived estimates of Wolbachia-mediated blocking of dengue virus transmission potential in Aedes aegypti mosquitoes. In: Proceedings of the National Academy of Sciences of the United States of America. 2017 ; Vol. 115, No. 2. pp. 361-366.
@article{9b787e60cb1f4771813c8a4ef8a3681e,
title = "Field- and clinically derived estimates of Wolbachia-mediated blocking of dengue virus transmission potential in Aedes aegypti mosquitoes",
abstract = "The wMel strain of Wolbachia can reduce the permissiveness of Aedes aegypti mosquitoes to disseminated arboviral infections. Here, we report that wMel-infected Ae. aegypti (Ho Chi Minh City background), when directly blood-fed on 141 viremic dengue patients, have lower dengue virus (DENV) transmission potential and have a longer extrinsic incubation period than their wild-type counterparts. The wMel-infected mosquitoes that are field-reared have even greater relative resistance to DENV infection when fed on patient-derived viremic blood meals. This is explained by an increased susceptibility of field-reared wild-type mosquitoes to infection than laboratory-reared counterparts. Collectively, these field- and clinically relevant findings support the continued careful field-testing of wMel introgression for the biocontrol of Ae. aegypti-born arboviruses.",
keywords = "Aedes aegypti mosquito, Dengue virus, Extrinsic incubation period, Virus transmission, WMel Wolbachia",
author = "Carrington, {Lauren B.} and Tran, {Bich Chau Nguyen} and Le, {Nhat Thanh Hoang} and Luong, {Tai Thi Hue} and Nguyen, {Truong Thanh} and Nguyen, {Phong Thanh} and Chau, {Nguyen Van Vinh} and Nguyen, {Huong Thi Cam} and Vu, {Trung Tuan} and Vo, {Long Thi} and Le, {Dui Thi} and Vu, {Nhu Tuyet} and Nguyen, {Giang Thi} and Luu, {Hung Quoc} and Dang, {Anh Duc} and Hurst, {Timothy P.} and O’Neill, {Scott L.} and Tran, {Vi Thuy} and Kien, {Duong Thi Hue} and Nguyen, {Nguyet Minh} and Marcel Wolbers and Bridget Wills and Simmons, {Cameron P.}",
year = "2017",
month = "1",
day = "9",
doi = "10.1073/pnas.1715788115",
language = "English",
volume = "115",
pages = "361--366",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "National Academy of Sciences",
number = "2",

}

Carrington, LB, Tran, BCN, Le, NTH, Luong, TTH, Nguyen, TT, Nguyen, PT, Chau , NVV, Nguyen, HTC, Vu, TT, Vo, LT, Le, DT, Vu, NT, Nguyen, GT, Luu, HQ, Dang, AD, Hurst, TP, O’Neill, SL, Tran, VT, Kien, DTH, Nguyen, NM, Wolbers, M, Wills, B & Simmons, CP 2017, 'Field- and clinically derived estimates of Wolbachia-mediated blocking of dengue virus transmission potential in Aedes aegypti mosquitoes', Proceedings of the National Academy of Sciences of the United States of America, vol. 115, no. 2, pp. 361-366. https://doi.org/10.1073/pnas.1715788115

Field- and clinically derived estimates of Wolbachia-mediated blocking of dengue virus transmission potential in Aedes aegypti mosquitoes. / Carrington, Lauren B.; Tran, Bich Chau Nguyen; Le, Nhat Thanh Hoang; Luong, Tai Thi Hue; Nguyen, Truong Thanh; Nguyen, Phong Thanh; Chau , Nguyen Van Vinh; Nguyen, Huong Thi Cam; Vu, Trung Tuan; Vo, Long Thi; Le, Dui Thi; Vu, Nhu Tuyet; Nguyen, Giang Thi; Luu, Hung Quoc; Dang, Anh Duc; Hurst, Timothy P.; O’Neill, Scott L.; Tran, Vi Thuy; Kien, Duong Thi Hue; Nguyen, Nguyet Minh; Wolbers, Marcel; Wills, Bridget; Simmons, Cameron P.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 115, No. 2, 09.01.2017, p. 361-366.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Field- and clinically derived estimates of Wolbachia-mediated blocking of dengue virus transmission potential in Aedes aegypti mosquitoes

AU - Carrington, Lauren B.

AU - Tran, Bich Chau Nguyen

AU - Le, Nhat Thanh Hoang

AU - Luong, Tai Thi Hue

AU - Nguyen, Truong Thanh

AU - Nguyen, Phong Thanh

AU - Chau , Nguyen Van Vinh

AU - Nguyen, Huong Thi Cam

AU - Vu, Trung Tuan

AU - Vo, Long Thi

AU - Le, Dui Thi

AU - Vu, Nhu Tuyet

AU - Nguyen, Giang Thi

AU - Luu, Hung Quoc

AU - Dang, Anh Duc

AU - Hurst, Timothy P.

AU - O’Neill, Scott L.

AU - Tran, Vi Thuy

AU - Kien, Duong Thi Hue

AU - Nguyen, Nguyet Minh

AU - Wolbers, Marcel

AU - Wills, Bridget

AU - Simmons, Cameron P.

PY - 2017/1/9

Y1 - 2017/1/9

N2 - The wMel strain of Wolbachia can reduce the permissiveness of Aedes aegypti mosquitoes to disseminated arboviral infections. Here, we report that wMel-infected Ae. aegypti (Ho Chi Minh City background), when directly blood-fed on 141 viremic dengue patients, have lower dengue virus (DENV) transmission potential and have a longer extrinsic incubation period than their wild-type counterparts. The wMel-infected mosquitoes that are field-reared have even greater relative resistance to DENV infection when fed on patient-derived viremic blood meals. This is explained by an increased susceptibility of field-reared wild-type mosquitoes to infection than laboratory-reared counterparts. Collectively, these field- and clinically relevant findings support the continued careful field-testing of wMel introgression for the biocontrol of Ae. aegypti-born arboviruses.

AB - The wMel strain of Wolbachia can reduce the permissiveness of Aedes aegypti mosquitoes to disseminated arboviral infections. Here, we report that wMel-infected Ae. aegypti (Ho Chi Minh City background), when directly blood-fed on 141 viremic dengue patients, have lower dengue virus (DENV) transmission potential and have a longer extrinsic incubation period than their wild-type counterparts. The wMel-infected mosquitoes that are field-reared have even greater relative resistance to DENV infection when fed on patient-derived viremic blood meals. This is explained by an increased susceptibility of field-reared wild-type mosquitoes to infection than laboratory-reared counterparts. Collectively, these field- and clinically relevant findings support the continued careful field-testing of wMel introgression for the biocontrol of Ae. aegypti-born arboviruses.

KW - Aedes aegypti mosquito

KW - Dengue virus

KW - Extrinsic incubation period

KW - Virus transmission

KW - WMel Wolbachia

UR - http://www.scopus.com/inward/record.url?scp=85040222797&partnerID=8YFLogxK

U2 - 10.1073/pnas.1715788115

DO - 10.1073/pnas.1715788115

M3 - Article

VL - 115

SP - 361

EP - 366

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 2

ER -