Field- and clinically derived estimates of Wolbachia-mediated blocking of dengue virus transmission potential in Aedes aegypti mosquitoes

Lauren B. Carrington, Bich Chau Nguyen Tran, Nhat Thanh Hoang Le, Tai Thi Hue Luong, Truong Thanh Nguyen, Phong Thanh Nguyen, Nguyen Van Vinh Chau , Huong Thi Cam Nguyen, Trung Tuan Vu, Long Thi Vo, Dui Thi Le, Nhu Tuyet Vu, Giang Thi Nguyen, Hung Quoc Luu, Anh Duc Dang, Timothy P. Hurst, Scott L. O’Neill, Vi Thuy Tran, Duong Thi Hue Kien, Nguyet Minh NguyenMarcel Wolbers, Bridget Wills, Cameron P. Simmons

Research output: Contribution to journalArticleResearchpeer-review

27 Citations (Scopus)

Abstract

The wMel strain of Wolbachia can reduce the permissiveness of Aedes aegypti mosquitoes to disseminated arboviral infections. Here, we report that wMel-infected Ae. aegypti (Ho Chi Minh City background), when directly blood-fed on 141 viremic dengue patients, have lower dengue virus (DENV) transmission potential and have a longer extrinsic incubation period than their wild-type counterparts. The wMel-infected mosquitoes that are field-reared have even greater relative resistance to DENV infection when fed on patient-derived viremic blood meals. This is explained by an increased susceptibility of field-reared wild-type mosquitoes to infection than laboratory-reared counterparts. Collectively, these field- and clinically relevant findings support the continued careful field-testing of wMel introgression for the biocontrol of Ae. aegypti-born arboviruses.

Original languageEnglish
Pages (from-to)361-366
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume115
Issue number2
DOIs
Publication statusPublished - 9 Jan 2017

Keywords

  • Aedes aegypti mosquito
  • Dengue virus
  • Extrinsic incubation period
  • Virus transmission
  • WMel Wolbachia

Cite this

Carrington, L. B., Tran, B. C. N., Le, N. T. H., Luong, T. T. H., Nguyen, T. T., Nguyen, P. T., Chau , N. V. V., Nguyen, H. T. C., Vu, T. T., Vo, L. T., Le, D. T., Vu, N. T., Nguyen, G. T., Luu, H. Q., Dang, A. D., Hurst, T. P., O’Neill, S. L., Tran, V. T., Kien, D. T. H., ... Simmons, C. P. (2017). Field- and clinically derived estimates of Wolbachia-mediated blocking of dengue virus transmission potential in Aedes aegypti mosquitoes. Proceedings of the National Academy of Sciences of the United States of America, 115(2), 361-366. https://doi.org/10.1073/pnas.1715788115