Fertdish: microfluidic sperm selection-in-a-dish for ICSI

Sa Xiao, Jason Riordon, Alexander Lagunov, Tom Hannam, Reza Nosrati, David Sinton

Research output: Chapter in Book/Report/Conference proceedingConference PaperOther

Abstract

The selection of viable sperm is critical for intracytoplasmic sperm injection (ICSI), an assisted reproductive technique. Existing conventional and microfluidic sperm selection methods, however, fail to select viable sperm and lack clinical applicability, respectively. Here, we present a clinically-applicable microfluidic platform, the FertDish, which improves upon the clinical ICSI petri dish by adding a two-layer film that includes an array of sperm-guiding micro-channels for one-step sperm selection. FertDish-selected sperm, from human donor semen samples, displayed considerable improvements in DNA quality when compared to raw semen. Notably, the FertDish outperforms conventional methods, and is readily applicable to the ICSI workflow.

Original languageEnglish
Title of host publicationMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages763-764
Number of pages2
ISBN (Electronic)9781733419017
Publication statusPublished - 2020
EventInternational Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2020) - Virtual, United States of America
Duration: 4 Oct 20209 Oct 2020
Conference number: 24th
https://microtas2020.org (Website)

Conference

ConferenceInternational Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2020)
Abbreviated titleMicroTAS 2020
CountryUnited States of America
Period4/10/209/10/20
Internet address

Keywords

  • Assisted Reproduction
  • Clinically-Applicable
  • Infertility
  • Microfluidics
  • Sperm Selection

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