Abstract
The selection of viable sperm is critical for intracytoplasmic sperm injection (ICSI), an assisted reproductive technique. Existing conventional and microfluidic sperm selection methods, however, fail to select viable sperm and lack clinical applicability, respectively. Here, we present a clinically-applicable microfluidic platform, the FertDish, which improves upon the clinical ICSI petri dish by adding a two-layer film that includes an array of sperm-guiding micro-channels for one-step sperm selection. FertDish-selected sperm, from human donor semen samples, displayed considerable improvements in DNA quality when compared to raw semen. Notably, the FertDish outperforms conventional methods, and is readily applicable to the ICSI workflow.
| Original language | English |
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| Title of host publication | MicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 763-764 |
| Number of pages | 2 |
| ISBN (Electronic) | 9781733419017 |
| Publication status | Published - 2020 |
| Event | International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2020) - Virtual, United States of America Duration: 4 Oct 2020 → 9 Oct 2020 Conference number: 24th https://microtas2020.org (Website) https://pubs.acs.org/toc/aamick/10/48?ref=breadcrumb (Proceedings) |
Conference
| Conference | International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2020) |
|---|---|
| Abbreviated title | MicroTAS 2020 |
| Country/Territory | United States of America |
| Period | 4/10/20 → 9/10/20 |
| Internet address |
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Keywords
- Assisted Reproduction
- Clinically-Applicable
- Infertility
- Microfluidics
- Sperm Selection