Abstract
Averaged estimates of RNA polymerase II (RNAPII) elongation rates in mammalian cells have been shown to range between 1.3 and 4.3 kb min -1. In this work, nascent RNAs from an integrated human immunodeficiency virus type 1-derived vector were detectable at the single living cell level by fluorescent RNA tagging. At steady state, a constant number of RNAs was measured corresponding to a minimal density of polymerases with negligible fluctuations over time. Recovery of fluorescence after photobleaching was complete within seconds, indicating a high rate of RNA biogenesis. The calculated transcription rate above 50 kb min -1 points towards a wide dynamic range of RNAPII velocities in living cells.
Original language | English |
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Pages (from-to) | 1280-1285 |
Number of pages | 6 |
Journal | EMBO Reports |
Volume | 12 |
Issue number | 12 |
DOIs | |
Publication status | Published - Dec 2011 |
Externally published | Yes |
Keywords
- FRAP
- HIV
- live imaging
- RNA polymerase II
- transcription