Fast targeted analysis of 132 acidic and neutral drugs and poisons in whole blood using LC-MS/MS

Matthew Di Rago, Eva Saar, Luke Neil Rodda, Sophie C Turfus, Alex Kotsos, Dimitri Gerostamoulos, Olaf Heino Drummer

Research output: Contribution to journalArticleResearchpeer-review

28 Citations (Scopus)

Abstract

The aim of this study was to develop an LC-MS/MS based screening technique that covers a broad range of acidic and neutral drugs and poisons by combining a small sample volume and efficient extraction technique with simple automated data processing. After protein precipitation of 100?L of whole blood, 132 common acidic and neutral drugs and poisons including non-steroidal anti-inflammatory drugs, barbiturates, anticonvulsants, antidiabetics, muscle relaxants, diuretics and superwarfarin rodenticides (47 quantitated, 85 reported as detected) were separated using a Shimadzu Prominence HPLC system with a C18 separation column (Kinetex XB-C18, 4.6mm?150mm, 5?m), using gradient elution with a mobile phase of 25mM ammonium acetate buffer (pH 7.5)/acetonitrile. The drugs were detected using an ABSciex? API 2000 LC-MS/MS system (ESI+ and -, MRM mode, two transitions per analyte). The method was fully validated in accordance with international guidelines. Quantification data obtained using one-point calibration compared favorably to that using multiple calibrants. The presented LC-MS/MS assay has proven to be applicable for determination of the analytes in blood. The fast and reliable extraction method combined with automated processing gives the opportunity for high throughput and fast turnaround times for forensic and clinical toxicology.
Original languageEnglish
Pages (from-to)35 - 43
Number of pages9
JournalForensic Science International
Volume243
DOIs
Publication statusPublished - 2014

Cite this

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title = "Fast targeted analysis of 132 acidic and neutral drugs and poisons in whole blood using LC-MS/MS",
abstract = "The aim of this study was to develop an LC-MS/MS based screening technique that covers a broad range of acidic and neutral drugs and poisons by combining a small sample volume and efficient extraction technique with simple automated data processing. After protein precipitation of 100?L of whole blood, 132 common acidic and neutral drugs and poisons including non-steroidal anti-inflammatory drugs, barbiturates, anticonvulsants, antidiabetics, muscle relaxants, diuretics and superwarfarin rodenticides (47 quantitated, 85 reported as detected) were separated using a Shimadzu Prominence HPLC system with a C18 separation column (Kinetex XB-C18, 4.6mm?150mm, 5?m), using gradient elution with a mobile phase of 25mM ammonium acetate buffer (pH 7.5)/acetonitrile. The drugs were detected using an ABSciex? API 2000 LC-MS/MS system (ESI+ and -, MRM mode, two transitions per analyte). The method was fully validated in accordance with international guidelines. Quantification data obtained using one-point calibration compared favorably to that using multiple calibrants. The presented LC-MS/MS assay has proven to be applicable for determination of the analytes in blood. The fast and reliable extraction method combined with automated processing gives the opportunity for high throughput and fast turnaround times for forensic and clinical toxicology.",
author = "{Di Rago}, Matthew and Eva Saar and Rodda, {Luke Neil} and Turfus, {Sophie C} and Alex Kotsos and Dimitri Gerostamoulos and Drummer, {Olaf Heino}",
year = "2014",
doi = "10.1016/j.forsciint.2014.03.021",
language = "English",
volume = "243",
pages = "35 -- 43",
journal = "Forensic Science International",
issn = "0379-0738",
publisher = "Elsevier",

}

Fast targeted analysis of 132 acidic and neutral drugs and poisons in whole blood using LC-MS/MS. / Di Rago, Matthew; Saar, Eva; Rodda, Luke Neil; Turfus, Sophie C; Kotsos, Alex; Gerostamoulos, Dimitri; Drummer, Olaf Heino.

In: Forensic Science International, Vol. 243, 2014, p. 35 - 43.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Fast targeted analysis of 132 acidic and neutral drugs and poisons in whole blood using LC-MS/MS

AU - Di Rago, Matthew

AU - Saar, Eva

AU - Rodda, Luke Neil

AU - Turfus, Sophie C

AU - Kotsos, Alex

AU - Gerostamoulos, Dimitri

AU - Drummer, Olaf Heino

PY - 2014

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N2 - The aim of this study was to develop an LC-MS/MS based screening technique that covers a broad range of acidic and neutral drugs and poisons by combining a small sample volume and efficient extraction technique with simple automated data processing. After protein precipitation of 100?L of whole blood, 132 common acidic and neutral drugs and poisons including non-steroidal anti-inflammatory drugs, barbiturates, anticonvulsants, antidiabetics, muscle relaxants, diuretics and superwarfarin rodenticides (47 quantitated, 85 reported as detected) were separated using a Shimadzu Prominence HPLC system with a C18 separation column (Kinetex XB-C18, 4.6mm?150mm, 5?m), using gradient elution with a mobile phase of 25mM ammonium acetate buffer (pH 7.5)/acetonitrile. The drugs were detected using an ABSciex? API 2000 LC-MS/MS system (ESI+ and -, MRM mode, two transitions per analyte). The method was fully validated in accordance with international guidelines. Quantification data obtained using one-point calibration compared favorably to that using multiple calibrants. The presented LC-MS/MS assay has proven to be applicable for determination of the analytes in blood. The fast and reliable extraction method combined with automated processing gives the opportunity for high throughput and fast turnaround times for forensic and clinical toxicology.

AB - The aim of this study was to develop an LC-MS/MS based screening technique that covers a broad range of acidic and neutral drugs and poisons by combining a small sample volume and efficient extraction technique with simple automated data processing. After protein precipitation of 100?L of whole blood, 132 common acidic and neutral drugs and poisons including non-steroidal anti-inflammatory drugs, barbiturates, anticonvulsants, antidiabetics, muscle relaxants, diuretics and superwarfarin rodenticides (47 quantitated, 85 reported as detected) were separated using a Shimadzu Prominence HPLC system with a C18 separation column (Kinetex XB-C18, 4.6mm?150mm, 5?m), using gradient elution with a mobile phase of 25mM ammonium acetate buffer (pH 7.5)/acetonitrile. The drugs were detected using an ABSciex? API 2000 LC-MS/MS system (ESI+ and -, MRM mode, two transitions per analyte). The method was fully validated in accordance with international guidelines. Quantification data obtained using one-point calibration compared favorably to that using multiple calibrants. The presented LC-MS/MS assay has proven to be applicable for determination of the analytes in blood. The fast and reliable extraction method combined with automated processing gives the opportunity for high throughput and fast turnaround times for forensic and clinical toxicology.

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