TY - JOUR
T1 - Fast standardized therapeutic-efficacy assay for drug discovery against tuberculosis
AU - Rullas, Joaquin
AU - Garcia, Juan I
AU - Beltran, Manuela
AU - Cardona, Pere-Joan
AU - Caceres, Neus
AU - Garcia-Bustos, Jose
AU - Angulo-Barturen, Inigo
PY - 2010
Y1 - 2010
N2 - Murine models of Mycobacterium tuberculosis infection are essential tools in drug discovery. Here we describe a fast standardized 9-day acute assay intended to measure the efficacy of drugs against M. tuberculosis growing in the lungs of immunocompetent mice. This assay is highly reproducible, allows good throughput, and was validated for drug lead optimization using isoniazid, rifampin, ethambutol, pyrazinamide, linezolid, and moxifloxacin.
AB - Murine models of Mycobacterium tuberculosis infection are essential tools in drug discovery. Here we describe a fast standardized 9-day acute assay intended to measure the efficacy of drugs against M. tuberculosis growing in the lungs of immunocompetent mice. This assay is highly reproducible, allows good throughput, and was validated for drug lead optimization using isoniazid, rifampin, ethambutol, pyrazinamide, linezolid, and moxifloxacin.
UR - http://aac.asm.org/content/54/5/2262.full.pdf
U2 - 10.1128/AAC.01423-09
DO - 10.1128/AAC.01423-09
M3 - Article
SN - 1098-6596
VL - 54
SP - 2262
EP - 2264
JO - Antimicrobial Agents and Chemotherapy
JF - Antimicrobial Agents and Chemotherapy
IS - 5
ER -
