Fast comprehensive two-dimensional gas chromatography with cryogenic modulation

Melanie Junge, Stefan Bieri, Helmut Huegel, Philip Marriott

Research output: Contribution to journalArticleResearchpeer-review

29 Citations (Scopus)

Abstract

The fast separation of a mixture of 29 compounds by using comprehensive two-dimensional gas chromatography is reported. Capillary column sets with shorter lengths and smaller inner diameter in both the first and second dimensions have been tested, for both fast chiral and achiral separations. Fast chiral separations, which included enantiomer separations of limonene, linalool, citronellol, and alpha-isomethylionone, were achieved within 23 min, which corresponds to similar to 2-fold faster than analyses under conditions previously considered as normal. Fast achiral separations, which do not have the restriction of requiring a minimum quality of chiral resolution, were obtained within 5 min, which is markedly faster than separations on the normal column set under conditions more commonly employed. The achiral fast GCxGC method used a 5 m x 0.1 mm i.d. first dimension column, interfaced to a 0.3 m x 0.05 mm i.d. second column, with temperature program rate of 35 degrees C.min(-1); a modulation period of 1 s was employed. Peak widths at baseline on the first column were a little over 1 s, while modulated peak widths at half-height recorded with a flame ionization detector operating at 200 Hz were similar to 30 ms. The benefits and limitations of GCxGC for fast chiral and achiral separations are reported and discussed.
Original languageEnglish
Pages (from-to)4448 - 4454
Number of pages7
JournalAnalytical Chemistry
Volume79
Issue number12
DOIs
Publication statusPublished - 2007
Externally publishedYes

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