Abstract
and tissue organization in vitro. Researchers have incorporated photodegradable
(PD) moieties into synthetic polymeric hydrogels as a means of
achieving spatiotemporal control over material properties. In this study
protein-based PD hydrogels composed of methacrylated gelatin and a
crosslinker containing o -nitrobenzyl ester groups are developed. The hydrogels
are able to degrade rapidly and specifi cally in response to UV light and
can be photopatterned to a variety of shapes and dimensions in a one-step
process. Micropatterned PD hydrogels are shown to improve cell distribution,
alignment, and beating regularity of cultured neonatal rat cardiomyocytes.
Overall this work introduces a new class of PD hydrogel based on
natural and biofunctional polymers as cell culture substrates for improving
cellular organization and function.
| Original language | English |
|---|---|
| Pages (from-to) | 977 - 986 |
| Number of pages | 10 |
| Journal | Advanced Functional Materials |
| Volume | 25 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2015 |
Cite this
}
Facile one-step micropatterning using photodegradable gelatin hydrogels for improved cardiomyocyte organization and alignment. / Tsang, Kelly M.C.; Annabi, Nasim; Ercole, Francesca; Zhou, Kun; Karst, Daniel J.; Li, Fanyi; Haynes, John M.; Evans, Richard A; Thissen, Helmut; Khademhosseini, Ali; Forsythe, John S.
In: Advanced Functional Materials, Vol. 25, No. 6, 2015, p. 977 - 986.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Facile one-step micropatterning using photodegradable gelatin hydrogels for improved cardiomyocyte organization and alignment
AU - Tsang, Kelly M.C.
AU - Annabi, Nasim
AU - Ercole, Francesca
AU - Zhou, Kun
AU - Karst, Daniel J.
AU - Li, Fanyi
AU - Haynes, John M.
AU - Evans, Richard A
AU - Thissen, Helmut
AU - Khademhosseini, Ali
AU - Forsythe, John S.
PY - 2015
Y1 - 2015
N2 - Hydrogels are often employed as temporary platforms for cell proliferationand tissue organization in vitro. Researchers have incorporated photodegradable(PD) moieties into synthetic polymeric hydrogels as a means ofachieving spatiotemporal control over material properties. In this studyprotein-based PD hydrogels composed of methacrylated gelatin and acrosslinker containing o -nitrobenzyl ester groups are developed. The hydrogelsare able to degrade rapidly and specifi cally in response to UV light andcan be photopatterned to a variety of shapes and dimensions in a one-stepprocess. Micropatterned PD hydrogels are shown to improve cell distribution,alignment, and beating regularity of cultured neonatal rat cardiomyocytes.Overall this work introduces a new class of PD hydrogel based onnatural and biofunctional polymers as cell culture substrates for improvingcellular organization and function.
AB - Hydrogels are often employed as temporary platforms for cell proliferationand tissue organization in vitro. Researchers have incorporated photodegradable(PD) moieties into synthetic polymeric hydrogels as a means ofachieving spatiotemporal control over material properties. In this studyprotein-based PD hydrogels composed of methacrylated gelatin and acrosslinker containing o -nitrobenzyl ester groups are developed. The hydrogelsare able to degrade rapidly and specifi cally in response to UV light andcan be photopatterned to a variety of shapes and dimensions in a one-stepprocess. Micropatterned PD hydrogels are shown to improve cell distribution,alignment, and beating regularity of cultured neonatal rat cardiomyocytes.Overall this work introduces a new class of PD hydrogel based onnatural and biofunctional polymers as cell culture substrates for improvingcellular organization and function.
UR - http://onlinelibrary.wiley.com/doi/10.1002/adfm.201403124/epdf
U2 - 10.1002/adfm.201403124
DO - 10.1002/adfm.201403124
M3 - Article
VL - 25
SP - 977
EP - 986
JO - Advanced Functional Materials
JF - Advanced Functional Materials
SN - 1616-301X
IS - 6
ER -