Facile one-step micropatterning using photodegradable gelatin hydrogels for improved cardiomyocyte organization and alignment

Kelly M.C. Tsang, Nasim Annabi, Francesca Ercole, Kun Zhou, Daniel J. Karst, Fanyi Li, John M. Haynes, Richard A Evans, Helmut Thissen, Ali Khademhosseini, John S. Forsythe

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67 Citations (Scopus)


Hydrogels are often employed as temporary platforms for cell proliferation
and tissue organization in vitro. Researchers have incorporated photodegradable
(PD) moieties into synthetic polymeric hydrogels as a means of
achieving spatiotemporal control over material properties. In this study
protein-based PD hydrogels composed of methacrylated gelatin and a
crosslinker containing o -nitrobenzyl ester groups are developed. The hydrogels
are able to degrade rapidly and specifi cally in response to UV light and
can be photopatterned to a variety of shapes and dimensions in a one-step
process. Micropatterned PD hydrogels are shown to improve cell distribution,
alignment, and beating regularity of cultured neonatal rat cardiomyocytes.
Overall this work introduces a new class of PD hydrogel based on
natural and biofunctional polymers as cell culture substrates for improving
cellular organization and function.
Original languageEnglish
Pages (from-to)977 - 986
Number of pages10
JournalAdvanced Functional Materials
Issue number6
Publication statusPublished - 2015

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