Facile one-step micropatterning using photodegradable gelatin hydrogels for improved cardiomyocyte organization and alignment

Kelly M.C. Tsang, Nasim Annabi, Francesca Ercole, Kun Zhou, Daniel J. Karst, Fanyi Li, John M. Haynes, Richard A Evans, Helmut Thissen, Ali Khademhosseini, John S. Forsythe

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Hydrogels are often employed as temporary platforms for cell proliferation
and tissue organization in vitro. Researchers have incorporated photodegradable
(PD) moieties into synthetic polymeric hydrogels as a means of
achieving spatiotemporal control over material properties. In this study
protein-based PD hydrogels composed of methacrylated gelatin and a
crosslinker containing o -nitrobenzyl ester groups are developed. The hydrogels
are able to degrade rapidly and specifi cally in response to UV light and
can be photopatterned to a variety of shapes and dimensions in a one-step
process. Micropatterned PD hydrogels are shown to improve cell distribution,
alignment, and beating regularity of cultured neonatal rat cardiomyocytes.
Overall this work introduces a new class of PD hydrogel based on
natural and biofunctional polymers as cell culture substrates for improving
cellular organization and function.
Original languageEnglish
Pages (from-to)977 - 986
Number of pages10
JournalAdvanced Functional Materials
Volume25
Issue number6
DOIs
Publication statusPublished - 2015

Cite this

Tsang, Kelly M.C. ; Annabi, Nasim ; Ercole, Francesca ; Zhou, Kun ; Karst, Daniel J. ; Li, Fanyi ; Haynes, John M. ; Evans, Richard A ; Thissen, Helmut ; Khademhosseini, Ali ; Forsythe, John S. / Facile one-step micropatterning using photodegradable gelatin hydrogels for improved cardiomyocyte organization and alignment. In: Advanced Functional Materials. 2015 ; Vol. 25, No. 6. pp. 977 - 986.
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abstract = "Hydrogels are often employed as temporary platforms for cell proliferationand tissue organization in vitro. Researchers have incorporated photodegradable(PD) moieties into synthetic polymeric hydrogels as a means ofachieving spatiotemporal control over material properties. In this studyprotein-based PD hydrogels composed of methacrylated gelatin and acrosslinker containing o -nitrobenzyl ester groups are developed. The hydrogelsare able to degrade rapidly and specifi cally in response to UV light andcan be photopatterned to a variety of shapes and dimensions in a one-stepprocess. Micropatterned PD hydrogels are shown to improve cell distribution,alignment, and beating regularity of cultured neonatal rat cardiomyocytes.Overall this work introduces a new class of PD hydrogel based onnatural and biofunctional polymers as cell culture substrates for improvingcellular organization and function.",
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Facile one-step micropatterning using photodegradable gelatin hydrogels for improved cardiomyocyte organization and alignment. / Tsang, Kelly M.C.; Annabi, Nasim; Ercole, Francesca; Zhou, Kun; Karst, Daniel J.; Li, Fanyi; Haynes, John M.; Evans, Richard A; Thissen, Helmut; Khademhosseini, Ali; Forsythe, John S.

In: Advanced Functional Materials, Vol. 25, No. 6, 2015, p. 977 - 986.

Research output: Contribution to journalArticleResearchpeer-review

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